作　　者： (郑梅霞）; (朱育菁）; (刘波）; (黄素芳）; (陈峥）; (张连宝）;

机构地区： 福建省农业科学院农业生物资源研究所,福建福州350003

出　　处： 《福建农业学报》 2017年第7期762-767,共6页

摘　　要： 为了获得更高品质的黄原胶,优化黄原胶高产菌株地毯草黄单胞菌FJAT-10151的发酵工艺,通过单因素及正交设计对培养基(碳源、氮源和无机盐离子)和发酵条件(发酵时间、pH、装液量和接种量)进行优化,试验获得最佳发酵培养基为葡萄糖30g·L^(-1)、豆饼粉30g·L^(-1)、KH2PO42g·L^(-1)、pH值9.0。在装液量50mL/250mL、接种量8%培养条件下发酵72h,黄原胶产量达到21.0g·L^(-1),是初始培养条件产量8.65g·L^(-1)的2.43倍。优化发酵工艺后,黄原胶品质提高,丙酮酸含量从8.9%升高到16.3%,而蛋白质含量从15.27%降低到4.8%。 Conditions of fermentation to produce extracellular xanthan gum by using Xanthomonas axonopodis FJAT-10151 were optimized to maximize quality of the gum.Single factor and orthogonal experiments on medium composition(including sources of carbon,nitrogen and inorganic ions)and fermentation conditions(including pH,time duration,fill volume and inoculum ratio)were conducted.It was found that using a medium consisting of glucose 30g·L^(-1),soybean cake powder 30g·L^(-1),and KH_2PO_4 2g·L^(-1) at pH 9.0with a filled culture of 50 mL containing 8% of inoculum per 250 mL flask,ayield of 21.0g·L^(-1) of xanthan gum could be obtained after fermentation for 72 h.The production capacity was 243% higher than that prior to the optimization.More importantly,a higher quality of xanthan gum with the content of pyruvic acid increased from 8.9% to 16.3% and the content of protein decreased from 15.27%to 4.8% was realized.

关 键 词： 地毯草黄单胞菌 黄原胶 正交设计