作　　者： (冯华）; (石尚友）; (罗秀琼）; (王世俊）; (郑凰雅）; (王样培）;

机构地区： 遵义市食品药品检验所中药室,贵州遵义563002

出　　处： 《甘肃中医药大学学报》 2017年第4期30-34,共5页

摘　　要： 目的建立苗药复方胃痛胶囊中吴茱萸的定性与定量分析方法。方法采用薄层色谱(TLC)法对苗药复方胃痛胶囊中的吴茱萸碱和吴茱萸次碱进行定性分析;采用高效液相色谱(HPLC)法,以Diamonsil C_(18)(4.6 mm×200 mm,5μm)为色谱柱,乙腈-水-四氢呋喃-冰醋酸(41∶59∶3∶0.1)为流动相,流速1.0 mL/min,检测波长225 nm,柱温30℃,测定苗药复方胃痛胶囊中吴茱萸碱和吴茱萸次碱的含量。结果所建立的TLC法斑点清晰、分离效果好。含量测定中吴茱萸碱和吴茱萸次碱的回归方程分别为Y=1 0 211X-6.838(r=0.999 7)和Y=1 931X-8.501(r=0.999 6),说明二者分别在0.098~0.783 mg/mL和0.068~0.544mg/mL范围内线性关系良好;二者的加样回收率分别为98.98%,97.78%。结论本法简便可行、重复性好,可用于苗药复方胃痛胶囊中吴茱萸的质量控制。 Objective To establish the qualitative and quantitative analysis methods for testing evodiamine in Fufang Weitong Jiaonang （ Miao medicine）. Methods Qualitative analysis was made to test evodiamine and rutae-carpine in the capsule by TLC;HPLC was used to determine the contents of evodiamine and rutaecarpine with the column of Diamonsil C 18 （4.6 mm×200 ram,5 μm）, moving phase was acetonitrile-waters-tetrahydrofuran-phospho- tic acid （41 ： 59 ： 3 ： 0.1 ） ,flow rate was 1.0 mL/min,detective wavelength at 225 nm and column temperature at 30℃. Results The spots were clear and separating effect was good. The regression equations of two elements were Y= 10 211X-6. 838 （r=0. 999 7 ）and Y= 1 931X-8. 501 （r=0. 999 6）, showing a good linearity in the range of 0. 098 -0. 783 mg/mL and 0. 068-0. 544 mg/mL;the recovery rates of two were 98.98% and 97.78% respec- tively. Conclusion The method is simple, effective with good repeatability and can be used for quality control of the capsules.

关 键 词： 苗药复方胃痛胶囊 吴莱萸碱 吴莱萸次碱 薄层色谱 高效液相色谱 分析