作　　者： (李聪丽）; (阳亚婕）; (傅本重）; (张志林）; (付应林）; (李国元）;

机构地区： 湖北工程学院生命科学技术学院/特色果蔬质量安全控制湖北省重点实验室,湖北孝感432000 湖北大学生命科学学院,湖北武汉430062

出　　处： 《河南农业科学》 2017年第9期73-78,共6页

摘　　要： 为明确香葱灰霉病病原及其生物学特性,采用单孢分离法分离香葱灰霉病病原菌,依据病原菌形态特征及r DNA-ITS序列分析结果,鉴定香葱灰霉病病原为灰葡萄孢菌(Botrytis cinerea Pers.),同时对其生物学特性进行研究。结果表明:病原菌在供试的10种培养基上均能生长,最适宜培养基为PDA培养基;供试的9种碳源和11种氮源中,最利于病原菌菌丝生长的碳源为可溶性淀粉,氮源为酵母粉;在pH值为4.0~12.0条件下,病原菌都能生长,最适pH值为5.0~6.0;病原菌在4~30℃均能生长,适合病原菌菌丝生长的温度为15~25℃,25℃时病原菌菌丝不仅生长快且非常浓密,温度高于30℃菌丝停止生长;菌丝致死温度是52℃(10 min)。 In order to identify the pathogen and its biological characteristics of Allium ascalonicum gray mould,the pathogen was isolated from diseased leaves of A. ascalonicum by single spore isolation method.Based on the morphological characteristics and r DNA-ITS sequence analysis,the pathogen of A.ascalonicum gray mould was identified as Botrytis cinerea Pers.. The biological characteristics of B. cinerea were studied,and the results showed that the pathogen was able to grow on the ten media tested,during which the optimum medium for the growth of the pathogen was PDA medium. In the nine carbon sources and eleven nitrogen sources tested,the optimum carbon and nitrogen sources were soluble starch and yeast powder respectively. The pathogen could grow in pH from 4. 0 to 12. 0,while the optimal pH was 5. 0—6. 0. The pathogen could grow at 4—30 ℃,and 15—25 ℃ was suitable for the mycelia growth. The mycelia grew quickly and very densely at 25 ℃,when the temperature was above 30 ℃,the mycelia growth stopped. The mycelia lethal temperature was 52 ℃ for 10 min.

关 键 词： 香葱 灰霉病 病原菌 生物学特性 灰葡萄孢菌