作　　者： (李瑞）; (侯改凤）; (韦良开）; (彭伟）; (潘杰）; (黄兴国）;

机构地区： 湖南农业大学动物科学技术学院,长沙410128 湖南畜禽安全生产协同创新中心,长沙410128

出　　处： 《动物营养学报》 2017年第9期3184-3192,共9页

摘　　要： 本试验旨在研究饲粮中添加德氏乳杆菌制剂对育肥猪血脂指标、肝脏胆固醇代谢和脂肪沉积相关酶活性、组织胆固醇代谢和脂肪沉积相关基因mRNA表达和皮下脂肪组织形态的影响。试验选用120头平均体重为(65.34±3.64)kg的健康"杜×(长×大)"育肥猪,随机分为2组,每组6个重复(栏),每个重复10头(公母各占1/2)。对照组饲喂基础饲粮,试验组饲喂在基础饲粮中添加0.10%德氏乳杆菌制剂的饲粮,预试期7d,试验期42d。结果显示:1)与对照组相比,试验组育肥猪的血清甘油三酯(TG)(P=0.08)和总胆固醇(TC)(P=0.06)含量均有降低趋势,血清总胆汁酸(TBA)含量显著降低(P<0.05)。2)与对照组相比,试验组育肥猪的肝脏羟甲基戊二酰辅酶A还原酶(HMGR)活性有降低趋势(P=0.07),胆固醇7α-羟化酶活性(CYP7A1)显著增加(P<0.05),脂肪甘油三酯脂酶(ATGL)活性极显著降低(P<0.01)。3)与对照组相比,试验组育肥猪的回肠胆汁酸结合蛋白(IBABP)的mRNA相对表达量有降低趋势(P=0.07),皮下脂肪过氧化物酶体增殖物激活受体γ(PPARγ)的mRNA相对表达量显著增加(P<0.05);皮下脂肪细胞直径有降低趋势(P=0.09)。结果提示,德氏乳杆菌可通过干扰育肥猪回肠对胆汁酸的吸收来调控肝脏胆固醇和脂肪代谢。 The experiment was conducted to study the effects of dietary Lactobacillus delbrueckii preparation on serum lipid indexes, enzyme activities in liver and genes mRNA expression in tissues related to cholesterol metabolism and fat deposition and histomorphology of subcutaneous fat of finishing pigs. One hundred and twenty healthy Duroc×（Landrace×Yorkshire） finishing pigs with average body weight of （65.34±3.64） kg were randomly divided into two groups with six replicates （pens） per group and ten pigs per replicate （males and females in half）. Pigs in the control group were fed a basal diet, while others in the experimental group were fed the basal diet supplemented with 0.10% Lactobacillus delbrueckii preparation. The pre-test lasted for 7 days and the experiment lasted for 42 days. The results showed as follows：1） compared with the control group, the contents of triglyceride （TG） （P=0.08） and total cholesterol （TC） （P=0.06） in serum of finishing pigs in the experimental group had a decrease trend, and the content of total bile acid （TBA） in serum was significantly decreased （P〈0.05）. 2） Compared with the control group, the activity of hydroxymethylglutaryl CoA reductases （HMGR） in liver of finishing pigs in the experimental group had a decrease trend （P=0.07）, the activity of cholesterol-7alpha-hydroxylase （CYP7A1） was significantly increased （P〈0.05）, and the activity of adipose triglyceride lipase （ATGL） was significantly decreased （P〈0.01）. 3） Compared with the control group, the mRNA relative expression of ileal bile acid binding protein （IBABP） of finishing pigs in the experimental group had a decrease trend （P=0.07）, and the mRNA relative expression of peroxisome proliferators activated receptor γ （PPARγ） in subcutaneous fat was significantly increased （P〈0.05）. Subcutaneous fat cell diameter had a decrease trend compared with the control group （P=0.09）. The results indicate that Lactobacillus delbrueckii

关 键 词： 德氏乳杆菌 育肥猪 酶活性 基因表达