作　　者： (崔丹丹）; (王傲杰）; (王新港）; (周峰）; (彭志锋）; (王川庆）;

机构地区： 河南农业大学牧医工程学院,河南郑州450002

出　　处： 《河南农业科学》 2017年第9期132-138,共7页

摘　　要： 为了解猪繁殖与呼吸综合征病毒(PRRSV)流行株的变异情况,将PRRSV阳性病料处理后接种Marc-145细胞,成功分离到1株PRRSV毒株,将其命名为HENZXC-4,并对其全基因组序列进行测定和分析。结果表明,HENZXC-4为未发生重组的类NADC30毒株,其NSP2蛋白存在131个氨基酸不连续缺失,且其GP5蛋白存在1个氨基酸插入。 To investigate the molecular variation characteristics of porcine reproductive and respiratory syndrome virus( PRRSV) in the field,a PRRSV isolate( HENXC-4) was isolated by inoculating the PRRSV positive samples into the Marc-145 cell layers. The whole genome was then sequenced and analyzed. The sequence analysis showed that HENXC-4 was NADC30-like PRRSV without any recombination phenomenon characterized by a discontinuous 131-amino-acid deletion in the NSP2 region.More importantly,it had a 1-amino-acid insertion in GP5,which was absent in other NADC30-like PRRSV strains.

关 键 词： 猪繁殖与呼吸综合征病毒 分离鉴定 类 株 序列分析