作　　者： (李玲玲）; (吴明雨）; (张宏）; (李坤）; (余孝其）;

机构地区： 绿色化学与技术教育部重点实验室四川大学化学学院,成都610064

出　　处： 《中国科学：化学》 2017年第8期1029-1034,共6页

摘　　要： 生物硫醇是一类非常重要的活性物质,在细胞的生长或凋亡过程中发挥着重要的作用.本文以香豆素为荧光团,吗啉为溶酶体靶向基团,成功设计并合成了生物硫醇的荧光识别探针Lyso-thiol.该探针可在磷酸盐缓冲溶液(PBS)体系中(pH 7.40)特异性地对生物硫醇产生"off-on"的荧光信号变化,具有高选择性与灵敏度,并可在较大浓度范围内对生物硫醇进行线性检测.在生物硫醇的饱和浓度下,探针最高可实现745倍的荧光强度增强.该探针还具有良好的生物相容性、溶酶体靶向能力及细胞成像能力. As an important member of bio-active species, biological thiols have been proved to play pivotal roles in cellular growth in cells. In this paper, we have designed and synthesized a new coumarin-based off-on fluorescent probe（Lyso-thiol） for the selective detection of biothiols in aqueous solution and in living cells. Lyso-thiol has been prepared by a 2-step procedure using morpholine as the lysosome-targeting group. The probe has successfully realized the linear quantitative detection of biothiols in a relatively large concentration scope. Moreover, under the saturated concentration of the biological thiols, the fluorescence intensity of the probe can be enhanced by as much as 745 times. The intracellular experiments have also confirmed the low cytotoxity and exceptional biocompatibility of the probe. It is validated both in HeLa and HepG 2 cells that Lyso-thiol is capable of specifically targeting lysosome and vividly imaging biothiols in cellular level.

关 键 词： 溶酶体靶向 荧光探针 硫醇检测 细胞成像