作　　者： (袁柳萍）; (罗霄山）; (孙冬梅）; (吴小斌）; (王洛临）;

机构地区： 广州中医药大学,广州510405 广东省中医药工程技术研究院广东省中医药研究开发重点实验室,广州510095

出　　处： 《世界中医药》 2017年第8期1918-1922,共5页

摘　　要： 目的:优选养阴安神颗粒的提取工艺条件。方法:通过观察养阴安神方对戊巴比妥钠催眠小鼠睡眠时间的影响,对该处方提取工艺进行了初步筛选;根据药效观察结果,以君药酸枣仁中斯皮诺素的提取率和固形物得率为评价指标,采用正交试验设计法,筛选出最优的水提工艺条件,并采用正交试验设计法,以斯皮诺素的保留率及固形物除杂率为评价指标,筛选出最佳醇沉工艺条件。结果:提取工艺路线采用全方水提醇沉的方法。水提最佳工艺为:提取3次,1 h/次,加8倍量水,醇沉最佳工艺为:水提液浓缩至相对密度1.25(60℃),放冷加入适量乙醇至乙醇含量为80%,4℃冰箱中静置36h。结论:优选的提取工艺稳定、可行,为养阴安神颗粒后期的成型工艺奠定了基础。 Objective： To optimize the extraction process of Yangyin Anshen granules. Methods： To observe the effect of Yangyin Anshen granules on sleeping of petobar-bital sodium mice in order to optimize the best extraction technololgy of Yangyin Anshen prescription. Pharmacological results shows that with composite score of extraction rate of spinosin which in the Gentleman medicine ziziphi spinosae semen and dry extract rate as comprehensive evaluation indexes,orthogonal test was used to optimize the water extracting technology. Then with composites score of retention rate of spinosin and impurity removing efficiency as comprehensive evaluation indexes,orthogonal test was used to optimize the alcohol precipitation technology. Results： Optimum water extraction technology conditions were as follows： boiled the herbs with eight times the amount of water for thrice,one hour for each time,and three times in total. Optimum alcohol precipitation technology conditions were as follows： condensed the relation density of the liquor as in 1. 25（ 60 ℃）,adding alcohol to 80% of alcohol concentration and depositing it in the refrigerator at 4 ℃ for 36 h. Conclusion： Optimized extraction technology is stable and feasible,which can provide basis for molding technology of Yangyin Anshen granules.

关 键 词： 养阴安神颗粒 提取工艺 正交试验