作　　者： (李亚南）; (严明）; (冯芳君）; (吴金红）; (徐小艳）; (范佩清）; (梅捍卫）;

机构地区： 上海海洋大学水产与生命学院,上海201306

出　　处： 《上海农业学报》 2017年第4期10-15,共6页

摘　　要： 水稻幼苗的中胚轴延长特性是影响旱直播出苗和早期幼苗活力的重要性状。水稻品种‘扎西马’与‘旱恢3号’杂交产生F_(2:3)家系群体,黑暗条件发芽幼苗的中胚轴长度呈连续分布,存在双向超亲。从具有极端表型的家系中各选1-2株中胚轴最长或最短的幼苗移植大田,分别提取DNA样品,各70个个体DNA等量混合构成2个混合DNA池。采用二代测序平台对混合DNA池进行约70×深度的全基因组重测序,集团分离分析检测到水稻第1、3、6、7、8、9、11、12染色体上18个染色体区域。根据亲本间以及长中胚轴品种光照处理的差异表达模式,推测LOC_OsO1g08220和LOC_Os08g15296等2个呈负调控模式的候选基因。 Mesocotyl elongation of rice seedlings is an important characteristics influencing seedling establishment and early seedling vigor after dry direct sowing. An F2：3 population was developed from the cross between the parental lines of ＇ Zhaxima＇ and ＇ Hanhui No. 3 ＇. The mesocotyl lengths of dark germinated seedlings had continuous distribution and transgression on both sides. One or two seedlings were selected from each F3 line with extremely long or short mesocotyl length. Two bulked DNA pools were prepared from DNA samples isolated from mesocotyl tissues of 70 seedlings with the longest（59-102 mm）or the shortest mesocotyls （2--20 mm） , respectively. The pooled DNA samples were sequenced by about 70 × coverage using the next generation genome sequencing platform. A total of 18 putative regions were declared on chromosome 1,3,6,7,8, 9,11 and 12. Based on differential expression between parental lines and in seedlings of long-mesocotyl parent after light treatment, two putative candidate genes （LOC_Os01g08220 and LOC_Os08g15296）were found to negatively regulate mesocotyl elongation.

关 键 词： 稻 中胚轴延长 集团分离分析 数量性状基因座 候选基因