作　　者： (陈明杰）; (宋晓霞）; (马丹丹）; (郭力刚）; (赵妍）; (王晨光）; (余昌霞）;

机构地区： 上海市农业科学院食用菌研究所、上海市农业遗传育种重点开放实验室、农业部南方食用菌资源利用重点实验室、国家食用菌工程技术研究中心,上海201403

出　　处： 《上海农业学报》 2017年第4期6-9,共4页

摘　　要： 以草菇菌丝恢复生长情况和基因指纹图谱方法,研究了采用甘露醇溶液低温保藏草菇菌种的效果。结果表明:不耐受低温的草菇菌种在甘露醇溶液的保护下,能够在4℃保藏后快速恢复生长,且10%甘露醇溶液的低温保藏效果要明显优于20%海藻糖溶液;遗传稳定性试验结果显示,经甘露醇低温保藏的草菇菌种的基因指纹图谱未发生改变。 The protective effect of mannitol on Volvariella volvacea strains stored at low temperature was investigated in terms of V. volvacea mycelia recovery growth and genetic fingerprinting. The results showed that under mannitol solution protection the low temperature-intolerant V. volvacea strains could quickly recover and grow after storage at 4 ℃, and the protective effect of 10% mannitol solution was much better than that of 20% trehalose solution. The test for genetic stability indicated that mannitol didn＇ t change the genetic characteristics of V. volvacea strains.

关 键 词： 草菇 恢复生长 基因指纹图谱 低温 甘露醇