作　　者： (鲍大鹏）; (白睿）; (高英女）; (李燕）; (王莹）;

机构地区： 国家食用菌工程技术研究中心、农业部南方食用菌资源利用重点实验室、上海市农业遗传育种重点开放实验室、上海市农业科学院食用菌研究所,上海201403

出　　处： 《上海农业学报》 2017年第4期1-5,共5页

摘　　要： 利用生物信息学方法从蛋白质基本理化特性、蛋白质三维结构以及蛋白表面电荷性质等方面,对灵芝中两种真菌免疫调节蛋白LZ-8和LZ-9进行了预测和比较分析。结果表明:LZ-9蛋白结构柔性较好,N端疏水性更强;利用同源建模构建的LZ-9三维结构模型具有稳定的空间立体结构。比较分析LZ-9与LZ-8蛋白表面静电势能,发现LZ-9中5个突变(R9G、K41Q、D45G、N58D和T89K)改变了蛋白表面静电性质,影响了LZ-9与细胞表面受体的结合,从而产生了与LZ-8不同的生物功能。 The basic physicochemical characteristics, 3-dimensional structure and electrostatic potential of two fungal immunomodulatory proteins LZ-8 and LZ-9 of Ganoderma lucidum were predicted and compared by bioinformatics methods. The results showed that LZ-9 has better flexibility, and the N-terminal a-helix of LZ-9 is more hydrophobic than LZ-8. The established 3-dimensional structure of LZ-9 has stable space spatial structure. The electrostatic potential analysis revealed that the mutations R9G, K41 Q, D45 G, N58D and T89k are located on the surface which interacted with the electric charged part of LZ-9, which impacts the binding to cell-surface receptor and produces different biometric feature.

关 键 词： 灵芝 理论预测 真菌免疫调节蛋白