作　　者： (胡立娟）; (刘慧萍）; (曾柳庭）; (廖海燕）; (张国民）; (杨凯麟）;

机构地区： 湖南中医药大学,湖南长沙410208

出　　处： 《中华中医药学刊》 2017年第9期2282-2284,I0013-I0016,共7页

摘　　要： 目的:研究补肾活血方对免疫性卵巢早衰小鼠PI3、Akt、Bcl2蛋白表达的影响,为临床治疗卵巢早衰提供思路。方法:以小鼠ZP3为抗原,皮下多点注射免疫BALB/c雌性小鼠建立免疫性POF模型。以补肾活血方低、中、高剂量进行治疗,同步设阳性对照组(补佳乐)和空白组(生理盐水)。给予相应药物。治疗21 d后采用HE染色法检测卵巢组织的病理变化,免疫组化方法检测卵巢颗粒细胞PI3K、Akt、Bcl2蛋白表达,并分析阳性表达的平均光密度。结果:模型组PI3K、Akt和Bcl2蛋白表达量明显减低,与空白组比较,差异具有统计学意义(P<0.01);各药物组干预后PI3K、Akt和Bcl-2蛋白表达量明显上调,与模型组比较,差异具有统计学意义(P<0.01);其中阳性药物组和高剂量组表达上调最为明显,且两组的效果等同。结论:补肾活血方改善卵巢功能的机制可能与上调颗粒细胞PI3、Akt、Bcl2的蛋白表达有关。 Objective： To observe the effect of Bushen Huoxue Recipe on PI3,Akt and bcl-2 protein expressions of immune ovarian failure mice and provide a train of thought. Methods： Mice ZP3 was used as antigen and the immune POF model was established by subcutaneous multi-point injection of immune BALB/c female mice. With Bushen Huoxue Decoction low,medium and high-dose treatment,synchronization of the positive control group（ progynova） and control group（ normal saline）,giving the corresponding drug. After 21 days of treatment,the pathological changes of ovarian tissue were detected by HE staining. The expressions of PI3 K,Akt and Bcl2 were detected by immunohistochemistry and the average light density of positive expression was analyzed. Results： In model group,PI3 K,Akt and Bcl-2 protein were significantly reduced. Compared with the normal group,the difference was statistically significant（ P〈0. 01）. The drug groups＇ PI3 K,Akt and Bcl-2 protein expressions were significantly upregulated and the difference was statistically significant（ P〈0. 01）. The positive drug group and high dose group＇s was up-regulated obviously and the two groups effects were equivalent. Conclusion： The mechanism of improving ovary function may be related to the increase of protein expressions of PI3,Akt and Bcl2 in granulosa cells.

关 键 词： 卵巢早衰 蛋白 凋亡