作　　者： (傅国英）; (徐雪梅）; (江素君）; (吕豪）;

机构地区： 衢州市中心血站,浙江衢州324002

出　　处： 《中国输血杂志》 2017年第7期836-837,共2页

摘　　要： 目的探讨速冻设备与速冻时间对制备冷沉淀质量的影响。方法采用MBF21速冻机与三洋-30℃低温冰箱对制备后冷沉淀分别在30、50 min时间段进行速冻;用全自动凝血分析仪测定不同设备、不同时间冰冻的冷沉淀中凝血因子FⅧ活性和纤维蛋白原含量,判断是否符合冷沉淀质量要求。结果 2种设备30 min内速冻的冷沉淀,纤维蛋白原含量测定均符合要求,合格率100%;FⅧ活性测定速冻机合格率97.5%,低温储血冰箱速冻的合格率62.5%,2者比较有统计学差异(P<0.01)。2种设备50 min速冻的冷沉淀,部分纤维蛋白析出,纤维蛋白原含量测定合格率均在50%以下,差异无统计学意义(P>0.05);FⅧ活性测定符合率明显降低,速冻机符合率为35%、低温储血冰箱符合率为12.5%,2者比较有统计学差异(P<0.05)。结论速冻机能使冷沉淀快速速冻,使冷沉淀质量更有保障;凝血因子FⅧ是不稳定的凝血因子,温度升高活性不断降低,制备时尽量缩短在冰箱外放置的时间,才能保证冷沉淀质量要求。 Objective To discuss the effect of quick freezer equipment and quick freezing time on the preparation of cryoprecipitate. Methods The cryoprecipitate, already prepared, was placed into the MBF21 freezer and minus 30℃ SANYO refrigerator for 30-minute and 50-minute storage. The activity of coagulation factor VIII, and the content of fibrinogen in different equipment were detected by automatic coagulation analyzer, in order to make sure whether it met the quality requirements.Results The content of fibrinogen in the cryoprecipitates from both of the equipment after 30-minute storage met the requirement, with the qualification rate of 100%.As to the activity determination of coagulation FVH, low temperature refrigerator showed a qualification rate of 62. 5%, which was significantly lower than that of quick freezer with a qualification rate of 97.5% （ P〈0. 01 ） .The cryoprecipitates from both of the equipment after 50-minute storage, with part of fibrin precipi- tation, had a qualification rate of fibrinogen content lower than 50% （ P〉0. 05 ） .The qualification rate of quick freezer and low temperature refrigerator was 35% and 12. 5%, respectively, with significant difference. Conclusion Quick freezer can make the cryoprecipitate quick-frozen, which can ensure the quality. The coagulation factor VIII is unstable, whose activity decreased with the increase of temperature.We should try our best to shorten the time off the cold chain.

关 键 词： 冷沉淀 速冻 时间 设备