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反相液相色谱法测定毛竹不同组织中的腐胺
Determination of putrescine in different tissues of bamboo by reversed-phase liquid chromatography

作  者: (时作龙); (李美琴); (王菲); (赵琼); (王冰); (徐丹丹);

机构地区: 兵器工业卫生研究所,陕西西安710065

出  处: 《中国卫生检验杂志》 2017年第15期2149-2151,共3页

摘  要: 目的通过对毛竹中腐胺年动态变化的研究,为毛竹抗旱研究提供参考。方法反相液相色谱法测定毛竹不同组织中的腐胺。结果毛竹组织中腐胺含量呈现多样的变化,总的来说各组织中腐胺含量为竹叶>竹枝>竹鞭,大小年毛竹多胺的变化呈现出不同的趋势,小年毛竹竹叶7月时含量较高,为11.39μg/g,随着时间推移腐胺的含量逐渐下降,进入春笋大年后腐胺含量增加;而小年竹枝中7月腐胺含量较低,为4.99μg/g,进入出笋大年后也呈现增加的趋势;竹鞭的腐胺含量7月最小,为2.50μg/g,次年4月出现大幅下降,含量仅为1.09μg/g。结论探索多胺对竹子笋芽分化的影响具有一定意义,多胺能够提高竹子的抗旱性,而且竹子能吸收外源多胺。将多胺作为生长调节物质研究竹子抗旱机理也是未来研究的方向。 Objective To provide a reference for the study on drought resistance of bamboo through the research on the annual dynamic change of putrescine in different tissues of bamboo. Methods The putrescine in different tissues of bamboo was determined by reversed phase liquid chromatography. Results The content of putrescine in bamboo tissue showed the diversity. In general,the putrescine content in different tissues was bamboo leaves bamboo branch bamboo whip. The changes of bamboo polyamines in bumper years and lunar years also showed different trends. In lunar year,small bamboo leaves had a high putrescine content of 11. 39 μg/g in July. The content of putrescine decreased gradually over time. The putrescine content increased when it entered into the bumper year of the bamboo shoots. The content of putrescine in bamboo branch had the lower content of4. 99 μg/g in July in lunar year,which began to increase when entering into bamboo shoot in the bumper year. The content of putrescine in bamboo whip was 2. 50 μg/g in July,which was the lowest. In the next April,there was a substantial decline with the content of only 1. 09 μg/g. Conclusion It is significant to explore the effect of polyamines on shoot differentiation of bamboo shoots. The polyamines can improve the drought-resistance of bamboo. The bamboo can absorb the exogenous polyamines.It is also the direction of the future research to study the drought resistance mechanism of bamboo that polyamine is used as the growth regulators.

关 键 词: 毛竹 腐胺 动态变化

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