作　　者： (杨蕙）; (刘林）; (杜青）; (李薇）; (赵洪庆）; (刘检）; (孟盼）; (王宇红）;

机构地区： 湖南中医药大学第一附属医院中心实验室,长沙410007

出　　处： 《中华行为医学与脑科学杂志》 2017年第8期684-688,共5页

摘　　要： 目的探讨地佐环平（dizocilpine，MK801）对糖尿病并发抑郁症大鼠抑郁样行为及海马神经元损伤的影响。方法建立糖尿病并发抑郁症大鼠模型，并采用随机数字表将其随机分为2组：模型组、MK801组，以健康SD大鼠为正常对照组，每组6只。采用旷场实验检测大鼠行为学，采用HE染色检查大鼠海马病理损伤；采用尼氏染色，检测大鼠海马神经元中的凋亡小体数目；采用TUNEL染色，观察大鼠海马神经元凋亡；采用免疫荧光法，测定Bax、Bcl-2凋亡蛋白的表达。结果与正常组比较，模型组大鼠海马活动次数显著减少，海马神经元空泡、尼氏小体减少、凋亡细胞增多，凋亡蛋白Bax表达显著增加（94．57±7．97，30．08±5．73，P〈0．01）而Bcl-2表达减少（24．65±5．26，57．93±7．85，P〈0．01）。与模型组比较，地佐环平组大鼠活动次数显著增加[（12．50±4．42）次，（5．17±2．04）次，P〈0．01]，海马病理损伤减轻，尼氏小体表达显著增加（133．55±16．74，67．51±19．32，P〈0．01）而凋亡细胞数量显著减少（22．50±6．35，42．00±6．20，P〈0．01），凋亡蛋白Bax表达减少而Bcl-2表达增加（33．00±4．57，24．65±5．26，P〈0．01）。结论地佐环平可能通过影响凋亡蛋白的表达，发挥保护糖尿病并发抑郁症大鼠海马神经元损伤的作用。 Objective To investigate the effects of dizocilpine（ MK801 ） on depressive-like behav- iors and damaged hippocampus in rats with diabetes-related depression.Methods The animal models of dia- betes-related depression were established and they were randomly divided into two groups based on random number table： model group and MK801 group,while 6 rats were included in each group. And another six health rats were regarded as control group. The Open-field test was used to detect the activities. The damage of hippocampus was valued by HE staining, Nissl staining, and Tunel staining. The protein expressions of Bax, Bcl-2 in hippocampus were detected by Western blot.Results The number of activities was significant decreased in Open-field test in model group when compared with control. Hippocampal neurons vacuoles, Nissl bodies were decreased and apoptotic cells were increased in hippocampus in model group as well. Fur- thermore,the expression of Bax was significant up-regulated（94.57±7.97 ,P〈0.01）, while the Bcl-2 was de- clined（24.65±5.26,P〈0.01 ）. Compared with the model group, the animals in MK801 group exhibited in- creased activities （ 12.50±4.42, P〈0.01 ）, which accompany with an increased Nissl body （ 133.55 ± 16.74, P〈 0.01） and a decreased apoptosis（22.50±6.35,P〈0.01）. Moreover,the expression of Bax was decreased andthe Bcl-2 was increased in MK801 group when they were contrasted to model（33.00±4.57,P〈0.01 ）. Conclusion MK801 is a significant element to regulate the expression of apoptosis protein including Bax, Bcl-2, and to protecte the hippocampal neuron in rats with diabetes-related depression effectively.

关 键 词： 糖尿病并发抑郁症 地佐环平 海马 凋亡