导　　师： 李艳华

学科专业： 090601

授予学位： 硕士

作　　者： ;

机构地区： 东北农业大学

摘　　要： 猪链球菌病是由多种不同群的链球菌引起的猪不同临床诊断类型传染病的总称,世界各地均有发生。它不仅可引起猪的败血症、脑膜炎、关节炎、肺炎、心内膜炎、多发性浆膜炎和局部脓肿等症状,同时还可导致生猪从业人员感染和死亡,是一种重要的人畜共患病。抗菌药物在预防和治疗猪链球菌病过程中起着重要的作用,但随着抗菌药物的广泛应用,猪链球菌菌株耐药性日趋严重,尤其对大环内酯类、四环素类、β-内酰胺类、磺胺类及喹诺酮类抗菌药物耐药性最为严重,而这些药物均为临床上治疗猪链球菌感染的传统有效药物。 如何防治猪链球菌病已经成为各国养猪业及科研人员共同关心的重要问题,目前,用于治疗猪链球菌病的抗菌药物给药方案是基于最小抑菌浓度的治疗策略。但是随着研究的不断深入,美国学者Zhao等指出了传统治疗策略中的弊端,提出选用抗菌药物和调整治疗方案应尽量关闭或缩小“突变选择窗”的新理论。本研究基于该理论,研究泰乐菌素和阿莫西林联合用药是否具有关闭或缩小“突变选择窗”的作用。 本试验以从东北三省/(黑龙江、辽宁、吉林/)猪场采集的100头猪鼻腔棉拭子为样品。通过K-B试验测定了100株猪链球菌对酒石酸泰乐菌素和阿莫西林钠的敏感性。结果表明,酒石酸泰乐菌素的耐药率为80/%;阿莫西林钠的耐药率为0/%,其中20/%的菌株对酒石酸泰乐菌素和阿莫西林钠均敏感,揭示了临床分离的猪链球菌对酒石酸泰乐菌素耐性性比较严重,而对阿莫西林钠则相对敏感。 以美国临床检验标准委员会/(NCCLS/)作为判断标准,采用微量稀释法和联合药敏试验分别测定了酒石酸泰乐菌素、阿莫西林钠、复方酒石酸泰乐菌素-阿莫西林钠对20株临床分离猪链球菌敏感株的MIC,并计算FIC指数。结果表明两药合用后,除三株� Swine streptococcicosis is a group of Infectious diseases of different diagnosis types caused by Streptococcus suis. As an important zoonosis pathogen, such bacteria can lead to not only the symptom of swine septicemia, meningitis, arthrophlogosis, pneumonia, endocarditis, polyserositis and focal abscess in swine, but also the infection and death in employed person of hog industry. Antibacterial agents have played an important role in preventing and treating streptococcicosis, yet with the widespread use of those medication, the drug resistance of Streptococcus suis is getting more and more serious, especially against macrolide, tetracyclines,β-lactam, Sulfanilamide and Quinolone antibiotics which were often used in treating Streptococcus.s infections tradtionally. It has become an important problem for pig-raising industry and scientific researchers of many countries about how to control swine streptococcicosis. At present, antibacterial strategy is the therapy based on the MIC. As the development of the research, American scholar Zhao. pointed the drawback of the traditional treatment strategy . A theory was pointed that the MSW should be closed or narrowed as far as possible when selecting and using antibacterial agents and adjusting treatment protocols. Based on the theory, we study if tyloain tartrate combined with amoxicillin sodium can have the effect of narrowing or closing the MSW. In the experiment, the samples were 100 swines’nasal swabs from hoggerys in the three provines of Northeast China. 100 strains of streptococcus suis sensitivity to tylosin tartrate and amoxicillin sodium were tested by K-B test. The result showed that, the resistant rate of tylosin tartrate was 80/%; the resistant rate of amoxicillin sodium was 0/%,20/% strains were sensitive to tylosin tartrate and amoxicillin sodium at the same time, Revealed that resistance of isolates of streptococcus suis was serious against tylosin tartrate and sensitive against amoxicillin sodium. By the United States Committee for

关 键 词： 猪链球菌 酒石酸泰乐菌素 阿莫西林钠 联合用药 突变选择窗 防突变浓度

领　　域： [农业科学] [农业科学] [农业科学]