导　　师： 吴德峰;庞海

学科专业： 090601

授予学位： 硕士

作　　者： ;

机构地区： 福建农林大学

摘　　要： 白细胞介素18是白细胞介素1家庭的一位独特的家族成员。它与白细胞介素1在结构上很相似,都是需要caspase-1才能使之前体变成有活性的因子。IL-18作为一种细胞因子可以诱生IFN-γ、增强NK细胞细胞毒性作用、增强NK细胞活性、增强Th1型免疫反应、抗肿瘤作用等多种生物功能。白介素18结合蛋白/(IL-18BP/)是新近发现的一种糖蛋白,属免疫球蛋白超家族成员,目前已发现有6种IL-8BP的同工蛋白。IL-18BP可以在体内外有效抑制IL-18的作用,因而被认为是IL-18的天然拮抗剂。 以往对IL-18和IL-18BP的研究分析只限于某个载体,没有专门针对原核系统、没有专门为了获取融合性蛋白而进行的。也没有在诱导表达和纯化的过程中,对IL-18和IL-18BP这两个天然拮抗剂进行专门的统一的分析,没有把他们的缓冲环境、融合蛋白诱导条件和洗杂、洗脱环境总结为共同的一个模式。IL-18和IL-18BP都存在于动物体内,因此对同一环境下的研究就显得十分有意义。本次实验对原核系统进行了表达和纯化,并以获得融合蛋白为目的,不断的探讨能够表达出融合蛋白的方法,同时对IL-18和IL-18BP表达、纯化条件进行比较,是一个纵向从PCR到纯化,横向从IL-18到IL-18BP的新的研究方法。它们在结构上虽然有一定程度的相似。但是还是相差很远,对它们表达和纯化的共同模式的摸索可能对它们作用机理、在动物体内的相互作用制衡的研究提供帮助。IL-18BP是IL-18的天然拮抗剂,由于他们都具有免疫的双向调节作用,所以在疾病的过程中通过两者的平衡,到达治疗和预防疾病的作用,那么本次实验的研究可能会对二者在共同的环境-动物体内平衡的研究具有一定程度上的意义。同时也将为基础兽医学和兽医免疫学提供新的科学研究依据,产生重要的影响。 实验研究采用PCR扩增出人白细胞介素18和人白细胞� IL-18 is a special member of the IL-1 family,which closely related to IL-1 in structure and require easpase-1 as an activing agent.Interleukin/(IL/)-18 was identified as an cell factor that induces the produce of interferon-gamma,enhances NK cell cytotoxicity,mprove the activity of natural killer cells as well as Thl immunoreation type and and fulls of antieancer effects and so on.Interleukin-18 binding protein/(IL-18BP/)is a novel glycoprotein and belongs to the immunoglobulin superfamily.So far,six IL-18BP isoforms have been found in various eDNA libraries.IL-18BP is regarded as a natural antagonist for IL-18 which efficiently inhibits the biological functions of IL—18 in vitro an d in vivo. Formerly,the research and analysis of IL-18 and the IL-18BP were only restricted in some vector,has not carry specially on almming at the nucleus system,and at gainning the fusion protein,also has not carry specially on unified analysis to these two natural antagonist compounds-IL-18 and IL-18BP in the induction expresses and purification,has not summarize mixed theirs cushion environment,inducting condition of the fusion protein,washes,the elution environment for a common pattern.IL-18 and IL-18BP exist in animal's vivo,therefore it appears very meaningful to research under the identical environment.The experiment uses analysis vertically and horizontally,longitudinal is form PCR to the purification,crosswise is form IL-18 to IL-18BP,although they has the certain similitude in structure,but their difference is very far,the scrabble between them that can use the common pattern is possibly to provide the help for their action mechanism,the research which keeps in balance in the animal in vivo's interaction. IL-18BP is the natural antagonist compound of IL-18,because they have the immune bidirectional adjust action,therefore in disease's process through beth's balanced,achieve the treatment and prevent disease's function,then this experiment's research is possibly to have the certain extent significance in the common e

关 键 词： 白细胞介素 白细胞介素 结合蛋白 原核系统 纯化 融合蛋白

领　　域： [生物学]