导　　师： 司军强

学科专业： 071003

授予学位： 硕士

作　　者： ;

机构地区： 石河子大学

摘　　要： 胆囊收缩素/(cholecystokinin,CCK/)是一种脑肠肽,不仅存在于消化系统,在中枢和外周神经系统也有广泛分布。在中枢神经系统中,CCK主要以硫酸化的八肽胆囊收缩素/(sulfated cholecystokinin octapeptide,CCK-8s/)作为主要活性成份发挥其生物学效应。CCK-8s参与许多重要的生理和病理生理过程,包括行为学、焦虑、学习//记忆过程、疼痛等。CCK-8s是目前发现最强的内源性抗阿片物质,还可以降低长期应用吗啡所产生的吗啡耐受。本实验运用免疫组织化学技术和细胞内微电极记录技术,观察了福尔马林致痛后对大鼠感觉传递通路背根神经节/(dorsal root ganglion,DRG/)和脊髓背角感觉神经元CCK免疫反应的影响,同时观察了CCK-8s对大鼠DRG神经元膜电位的作用。所得结果如下: 1、福尔马林致痛对大鼠DRG神经元CCK免疫反应阳性细胞率的影响 大鼠足底注射生理盐水1 h后,注射侧和非注射侧仅有少数DRG的小神经元和大、中神经元CCK免疫反应呈弱阳性。非注射侧和注射侧DRG小神经元CCK免疫反应阳性细胞率分别为24.66/%±4.81/%和26.21/%±4.23/%,大、中神经元免疫反应阳性细胞率分别为3.99/%±1.75/%和4.44/%±1.54/%,差异均无显著性。 大鼠足底注射福尔马林1 h后,注射侧DRG小神经元CCK免疫反应阳性率为60.37/%±6.92/%,大、中神经元免疫反应阳性率为26.96/%±4.01/%,较同侧生理盐水1 h组均显著增加。非注射侧DRG小神经元CCK免疫反应阳性率为25.22/%±5.08/%,与同侧生理盐水1 h/(24.66/%±4.81/%/)相比,差异无显著性;而大、中神经元免疫反应阳性率为11.73/%±2.73/%,较同侧生理盐水1 h组/(3.99/%±1.75/%/)相比,差异有显著性。注射侧与非注射侧相比,大、中神经元和小神经元CCK免疫反应阳性率差异均有显著性。 在大鼠足底注射福尔马林3 h后,注射福尔马林侧DRG小神经元CCK免疫反应阳性率为49.78/%±3.68/%,大� CCK is a kind of brain-gut peptide that is abundantly distributed in both gastrointestinal tract and the central and peripheral nervous systems. In the central nervous system, the sulfated octapeptide fragment of cholacystokinin /(CCK-8s/) is engaged in numerous physiological and pathological processes including behavior, anxiety, learning//memory processes and pain. CCK-8s is one of the strongest endogenous anti-opioid substances and suppresses morphine tolerance which results from long term use of morphine. Here we observe the effect of formalin-induced pain on CCK immunoreactivity in rat sensory neurons through immunohistochemistry technique, and test the effect of CCK-8s on membrane potential of DRG neurons through intracellular recording technique. Results are as follows: 1. The Effect of Formalin-induced Pain on positive rate of CCK Immunoreactivity in Rat DRG Neurons. After 1h of subcutaneous injection of saline in one paw of rats, we observe that CCK immunoreactivity in few small, moderate and big neurons on DRG are weak positive both in injection and non-injection side. Positve rate of CCK immunoreactivity of small neurons on DRG are 24.66/%±4.81/% and 26.21/%±4.23/% in injection and non-injection respectively/(t=0.22,P>0.05/); and positve rate of CCK immunoreactivity of moderate and big neurons on DRG are 3.99/%±1.75/% and 4.44/%±1.54/% respectively/(t=0.16, P>0.05/), no remarkable difference reveals. After 1h of subcutaneous injection of formalin in one paw of rats, positive rate of CCK immunoreactivity of small neurons on DRG is 60.37/%±6.92/% in injection side which is greater than that of saline group/(t=4.21,P<0.01/). and positive rate of CCK immunoreactivity of moderate and big neurons on DRG is 26.96/%±4.01/%, higher than that of saline group /(t=5.24, P<0.01/). In non-injection side, positive rate of CCK immunoreactivity of small neurons on DRG is 25.22/%±5.08/%, no differences reveal compared with saline group/(t=0.08,P>0.05/); positive rate of CCK immunoreacti

关 键 词： 免疫组织化学 感觉信息 脊髓背角 细胞内记录 免疫反应性

领　　域： [生物学] [生物学]