导　　师： 曾哲灵;熊涛

学科专业： 071005

授予学位： 硕士

作　　者： ;

机构地区： 南昌大学

摘　　要： 共轭亚油酸/(CLA/)为亚油酸/(LA/)的异构体，具有抗癌、降脂、调节免疫、抗粥样硬化等重要的生理功能，目前共轭亚油酸/(CLA/)的生产大都是利用碱催化亚油酸异构而成，但其异构体繁多。利用微生物转化生产的共轭亚油酸/(CLA/)，异构体较少，与天然食品中的CLA异构体组成相似，具有很好的商业前景。 本论文采用紫外分光光度法从酸菜汁中分离产共轭亚油酸/(CLA/)乳酸菌，采用伯杰氏系统细菌分类法进行菌种鉴定，采用紫外线诱变和硫酸二乙酯诱变选育共轭亚油酸/(CLA/)高产菌。其主要研究内容为：从酸菜汁中筛选产CLA乳酸菌、菌种的鉴定、菌种的紫外线和硫酸二乙酯诱变选育、发酵培养基和发酵条件的优化。结果表明： 1、自酸菜汁中分离出的产共轭亚油酸/(CLA/)能力较强的乳酸菌X11，为植物乳酸杆菌/(Lactobacillus plantarum/)。 2、L.plantarumX11经紫外线诱变和硫酸二乙酯诱变选育出的共轭亚油酸/(CLA/)高产菌L.plantarumX11-3-1，产共轭亚油酸/(CLA/)的水平为0.342mg／ml，将亚油酸转化共轭亚油酸/(CLA/)的转化率为37.9％。最佳培养基组成为：葡萄糖、蛋白胨、酵母粉、牛肉膏、柠檬酸二铵、乙酸钠、磷酸氢二钾、硫酸镁、吐温80；它们的含量/(w／v/)分别为3％、1％、0.5％、1％、0.1％、0.5％、0.2％、0.02％、0.12％；最佳发酵条件为：培养基中亚油酸的含量为0.1％/(v／v/)，接种量为2％，发酵温度为34℃，发酵液初始pH值为6.5，静置发酵时间为36h。 Conjugated linoleic acid/(CLA/)is the isomer of linoleic acid/(LA/),which has a noveltype of biologically beneficial functional including reduces carcinogenesis,reducesbody fat,immunity regulation,and anti-atherosclerosis. Presently,CLA is producedthrough chemical isomerization of linoleic acid/(LA/),but products contain severalisomers. CLA isomers are much simpler through biological isomerization of LA thanthrough chemical. And the isomers are similar with the nature food. It has a goodbusiness future for CLA which is isomerized by microorganism. CLA production lactobacillus were screened from pickle juice through UVspectrophotometry, and were identified according to Bergey's Manual of SystemaicBacteriology.High-yielding CLA lactobacillus was indued by ultraviolet tradiationand diethyl sulfate/(DES/) mutant.The process of CLA production by Lactobacillusplantarum were studied. The research include screening, identification inducedmutation, fermentated,culture optimization and optimal condition of fermentation. Thestudy content includes: First, Lactic acid bacteriaX11, with a high ability to produce CLA,was isolatedfrom pickle juice was identified as a strain of Lactobacillus plantarum. Secongd, L. plantarumX11 was indued by ultraviolet tradiation and DES,a mutationstrain L. plantarumX11-3-1 was screened out with the highest production of CLA.Theoptimal fermented culture contents contains /(w//v/):glucose /(3/%/),peptone /(1/%/),yeastextract/(0.5/%/),beef extract/(1/%/), citric acid diamine /(0.1/%/), sodium acetate/(0.5/%/),potassium dihydrogen phosphate/(0.2/%/),magnesium sulfate/(0.02/%/),Tween80/(0.12/%/).The CLA concentration was 0.342mg//ml in optimal fermented culture pH6.5which was added 0.1/% /(v//v/)linoleic acid and was fermented 36h at 34℃.The yield ofCLA is 37.9/%.

关 键 词： 共轭亚油酸 亚油酸 植物乳杆菌 选育

领　　域： [生物学]