导　　师： 陈翔;陈迪

学科专业： 080903

授予学位： 硕士

作　　者： ;

机构地区： 上海交通大学

摘　　要： 磁珠/(Magnetic Beads/)技术具备固相化试剂的特点以及免疫反应的特异性,因其比表面积及偶联容量大、非接触式操控简单方便等优势,近年来在生物医学工程中得到了广泛的应用。结合微流控技术的基于磁珠的微分离芯片系统,在微全分析系统中已日益成为研究热点,在细胞分离,免疫反应以及DNA提取等生物分析领域都有着可观的应用前景。 本论文系统研究了一种新型的基于磁珠技术的微流控生物分离芯片系统,建立了一套从理论建模、模拟分析到制作封装的系统构建,并进行了磁珠在片实验。 本论文的主要工作有: 1.概述了磁珠微流控分离系统及在生物医学工程中的应用,总结了磁珠微系统化方面的研究进展,提出了自己的研究方案; 2.在对静磁学理论及层流理论进行研究归纳的基础上,分析了磁珠微系统的工作原理,对设计的磁珠微系统进行建模,进行磁场模拟分析,建立了磁场捕获的可行性分析。 3.设计了磁珠微系统结构及工艺流程,制作了基于玻璃基底的镍铁合金微电铸工艺和PDMS-玻璃永久键合工艺的全透明的磁珠微系统,并开发了微芯片系统的宏微接口工艺。 4.进行了磁珠微系统的在片实验,对该系统进行了功能性和应用性的验证,成功的实现了磁珠的在片操控,同时进行了蛋白,DNA等生物样品的在片实时分离反应。 本论文工作初步系统建立了基于磁珠的生物微分离芯片系统的方法,此系统具有敏感性高、所需样品量少、操作简单等优点,是生物医学工程中一种具有很好应用前景的新技术。 Magnetic Bead /(MB/) is widely used in biomedical engineering for its high surface area, coupling capacity and convenience of non-touch manipulation. Combined with microfluidic technologies, research on MB-based bio-microsystem has become one of the most attractive fields inμ-TAS, which can be widely used in cell separation, immunoassay and DNA extraction. This dissertation focuses on a novel MB bio-microsystem based on microfluidic technology. The topics of this dissertation are presented below: 1. Introduction of MB technology and its applications in biomedical engineering. Eliciting research scheme based on the investigation of achievements concerning MB microsystem. 2. Theory of the MB microsystem was studied. Modeling and Simulation of the magnetic field regenerated by the permalloy microelements was performed. The feasibility of magnetic capture has also been discussed. 3. Process design of the MB microsystem. A fully transparent microsystem based on permalloy micro-electroplating and PDMS//Glass bonding was well constructed and a macro-to-micro interface process was developed for the system. 4. On-chip experiments. On-chip operation of magnetic beads has been testified successfully and some experiments have been developed to realize the real-time, on-chip separation of some biological samples including proteins andλDNA products. The dissertation primarily provides a systematic approach towards the MB bio-microsystem based on microfluidic technologies.

关 键 词： 微磁珠 镍铁合金 磁场模拟 微流控

领　　域： [电子电信]