导　　师： 蔡俊鹏

学科专业： 083201

授予学位： 硕士

作　　者： ;

机构地区： 华南理工大学

摘　　要： 蛭弧菌是一类专门以攻击、进而裂解其他细菌为生的寄生性细菌。能裂解大肠杆菌、沙门氏菌等多种常见的细菌，在水体净化、水产养殖、家禽养殖等方面有成功的应用。作为一种生物净化因子，蛭弧菌具有良好的寄生特性、宿主范围广和无残留的优点，有应用于食品安全的可能。 本采用选择性培养基即培养基无有机营养成分培养法，只提供蛭弧菌可裂解的宿主菌，这样可以遏制其他杂菌的生长，达到纯化蛭弧菌的作用。最后将纯化好的蛭弧菌进行室发酵扩大培养，游泳体形式用于消除。 以草鱼鱼片为实验材料，为了有效控制这类生食食品携带致病菌的生长，比较了物理（超高压）、化学（双氧水）和生物（蛭弧菌M01）三种方法对草鱼片上接种的猪霍乱沙门氏菌进行消除和控制的效果，并对被处理鱼片进行了感官评定。 结果表明，与对照组相比，三种方法对草鱼片猪霍乱沙门氏菌均有很好的控制作用/(p<0.05/)；且蛭弧菌控制猪霍乱沙门氏菌的生物方法优于超高压和双氧水的物理、化学方法。与其他两种方法相比，蛭弧菌M01处理鱼片的生物方法能在较长时间内（48h）控制草鱼片猪霍乱沙门氏菌的增长，保持鱼片良好的感官状态。虽然在25℃保存//保鲜期间，生物处理组的猪霍乱沙门氏菌的初始浓度高于其它两种方法的处理组，但其增长受到更大的控制。结果表明，在受低度污染的鱼片保鲜中，蛭弧菌技术具有潜在的应用价值。 Bdellovibrio are predators of other bacteria. It can lyse some common bacteria, for example E.coli, salmonella and so on. Reports on Bdellovibrio sp was successfully used in waterpurification, aquaculture, poultry breed and medical specialty were common. As a kind ofliving creaster decontamination factor，Bdellovibrio has good parasitism, wide host range andno residue. It will be a wise choice of using Bdellovibrios to eliminate or suppress thepathogens in food. The selective medium without organic nutrients was applied and the host bacteria wasthe only prey for the Bdellovibrio to lyse in the study. Then the other bacteria was curbed andthe Bdellovibro was purified. Finally, the purified Bdellovibrio was fermented to expand inthe laboratory and applied in the elimination experiment. In order to control growth of pathogen on the uncooked or half-cooked food like sashimi,effectiveness and availability of physical /(HPP/), chemical /(H2O2/) and biological methods/(Bdellovibrio bacteriovorus M01/) were compared in controlling growth of Salmonella entericCholeraesuis /(S.Choleraesuis/) on grass carp fillets. The results showed that all of the three methods caused a significantly reduction of thispathogenic bacterium /(p<0.05/); In the lysis experiment, the effect of biological method is thebest and the low-dose group; In the sensory assessment, biological method was better thanphysical and chemical methods in all the48hours and ensure sensory attribute of the fillets.During storage at25℃, although with a higher original bacterium population than the othertwo methods, BDM01performed better in controlling bacterial growth. The results indicatedBALO /(Bdellovibrio-and-like organisms/) possess potential application value in fillets storagewith low contamination.

关 键 词： 草鱼片 猪霍乱沙门氏菌 蛭弧菌 超高压

分 类 号： [TS254.4]

领　　域： [轻工技术与工程] [轻工技术与工程]