导　　师： 胡卫列

学科专业： 100210

授予学位： 硕士

作　　者： ;

机构地区： 南方医科大学

摘　　要： Objective To investigate the expressions of nuclear transcription facrorκB/(NF-κB/) and inducible nitric oxide synthase/(iNOS/) in adrenal disease,and to study the roles in the course of adrenal disease and clinical significance. Methods and results 1:The acquisition of Adrenal cortical hyperplasia, cortical adenomas and normal adrenal tissue and the extraction of total RNA In aseptic conditions, collect all the specimens in the freezing tube and stored at-196℃liquid nitrogen. A total of access to adrenal hyperplasia in 22 cases, adrenal cortical adenoma in 14 cases,12 cases of normal adrenal tissue.Isolate total RNA from tissues by Trizol instructions. Results:Case group specimens were confirmed by pathology. Specimens were obtained for total RNA each other.. 2.RT-PCR detection of NF-κB and iNOS gene expression Reverse transcription of cDNA from the total RNAof 48 specimens, and then take the cDNA products to PCR amplified, carried out by agarose gel electrophoresis Results:In the human adrenal cortex hyperplasia and adenoma tissue the expression of positive rates of NF-κB and iNOS mRNA were significantly higher than the normal group /(P<0.05/);There was no significant difference in the rate between the adrenal cortex hyperplasia and adenoma /(P> 0.05/), 3.Quantitative PCR detection of NF-κB and iNOS gene expression Take the total RNA samples of 48 cases to quantitative RT-PCR reaction product, using samples fitting analysis results,obtaine the threshold cycle number /(Ct/) values of NF-κB,iNOS andβ-actin. Results:The mRNA levels of NF-κB,iNOS in The human adrenal cortex hyperplasia and adenoma tissue were significantly higher than the normal group /(P <0.05/).There was no significant difference between the adrenal cortex hyperplasia and adenoma /(P> 0.05/),the expression of NF-κB,iNOS mRNA is positive Correlation/(r=0.984 P<0.05/) 4.the acquisition of Immunohistochemical specimens and the NF-κB and iNOS protein expression in every gro

关 键 词： 肾上腺皮质增生 肾上腺皮质腺瘤 嗜铬细胞瘤 核转录因子 诱导型一氧化氮合酶

领　　域： [农业科学] [农业科学]