作　　者： ; ; ; ; ; ; ; ; ;

机构地区： 军事医学科学院放射与辐射医学研究所

出　　处： 《军事医学科学院院刊》 2004年第2期120-122,共3页

摘　　要： 目的 :通过电磁脉冲 (EMP)辐照成年犬 ,长期动态观察角膜、晶状体及视网膜的病理变化 ,探讨EMP对视觉系统的影响。方法 :高场强 (2～ 12 )× 10 4V/m的EMP、1～ 30次辐照实验犬 ,采用Feulgen ,AgNOR和PI染色方法 ,以激光扫描共聚焦显微镜 (LSCM)和图像分析技术进行检测。结果 :EMP辐射可使晶状体上皮细胞AgNOR和DNA含量下降 ,前皮质水肿 ,白内障发生。对EMP损伤敏感程度依次为 :晶状体 >角膜 >视网膜。损伤具有剂量 效应相关性。结论 :高场强EMP可引起犬晶状体前皮质混浊、白内障发生 ,损伤具有剂量 效应相关性。 Objective: To study the effect of EMP on optical system of dogs irradiated with EMP, and to observe microscopically the pathological changes in their cornea, lens and retina. Methods: Dogs were irradiated with (2-12)×10 4 V/m of EMP for 1 to 30 times. Feulgen, AgNOR and PI staining, LSCM and image analysis were used to detect and analyze the pathological changes. Results: The proliferative activity of lens epithelial cells was decreased by EMP radiation. Anterior capsular edema and cataract happened subsequently. The sensitivity to EMP injury were as follows: lens>cornea>retina. The injurious effect was dosage-related. Conclusion: High power EMP may cause anterior capsular edema of lens and cataract subsequently, and the injurious effect was dosage-related.

关 键 词： 电磁脉冲 犬 激光扫描共聚焦显微镜 晶状体 白内障

领　　域： [理学] [理学]