机构地区: 华南师范大学化学与环境学院化学系
出 处: 《海南大学学报(自然科学版)》 2004年第2期123-129,共7页
摘 要: 在pH=2.68的缓冲溶液中,茜素红与牛血清白蛋白发生了相互作用,研究表明:二者主要靠静电作用力结合,且它们的结合符合Pesavento提出的相分配模型.根据相分配模型,推导了有机小分子在蛋白质微相和水相中的分配比D的计算公式,并通过吸光度A、分配比D、表观结合常数K和热力学参数ΔG、ΔH、ΔS讨论了系列浓度下的阴离子型表面活性剂十二烷基硫酸钠(SDS)对该体系的影响,认为SDS主要通过静电作用力和疏水作用力影响茜素红与BSA的相互作用. Interaction of alizarin red(AR)and bovine serum albumin(BSA)is investigated in the buffer solutions at pH 2.68.It is considered that the combination of AR and BSA is due to the electrostatic force and the'Phase Distribution Model'is the proper description of the interaction between AR and BSA.According to the assumption of Pesavento,the distribution ratio D of AR between 'protein phase'and'water phase'is obtained.It is studied that the effection of sodium dodecyl sulphate (SDS) at different concentrations on the interaction between AR and BSA is mainly due to the electrostatic force and hydrophobic force by discussing the relate parameters.