作　　者： ; ; ; ; ;

机构地区： 南京师范大学化学与环境科学学院

出　　处： 《微生物学通报》 2004年第3期79-84,共6页

摘　　要： 从土壤中分离到一株降解2,4-二氯酚能力较强的假单胞菌菌株GT241-1,从中克隆出参与降解2,4-二氯酚的双烯内酯水解酶基因(dcpD)。该基因编码的双烯内酯水解酶可将顺式-2-氯双烯内酯水解成2-氯马来乙酸。采用的基因克隆策略是用Southern杂交对其邻近基因进行定位后构建基因组文库,再用斑点杂交筛选目的转化子。经序列测定得知dcpD基因编码区702bp。核苷酸和推测编码的氨基酸序列分析表明,dcpD与已在GenBank登记的相关基因有一定的差异。 A 2,4 -dichlorophenol degrading Pseudomonas strain GI241-1 was isolated from a soil sample. The dienelactone hydrolase gene, designated as dcpD which encodes dienelactone hydrolase involved in transforming cis-2-chloro-dienelactone into 2-chloromaleylacetic acid, was cloned from this bacterium strain. The gene cloning strategy was to construct genomic library after location of its neighbouring gene by Southem blot and to screen the aim transformant by dot blotting. Sequencing results showed that length of dcpD is 702bp. The sequence of dcpD and the deduced amino acid are different from the relative sequences registered in the GenBank.

关 键 词： 假单胞菌 二氯酚 双烯内酯水解酶基因 基因克隆

领　　域： [生物学]