作　　者： ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《细胞生物学杂志》 2004年第3期324-326,共3页

摘　　要： 利用尼龙膜作为介质,以0.4 mol/L的NaOH为层析液,进行膜上层析,分离经放射性同位素标记的核酸探针和未掺入的放射性游离单核苷酸,再经放射性强度测定即可计算出标记后的核酸探针的放射性比活。方法简单快捷,产生的放射性废物少,完全可以替代经典的三氯乙酸沉淀法及滤膜吸附法。 We reported here a new method for detection of radioactivity of radiolabeled nucleic acid probes. In this method, nylon filter and 0.4 mol/L of NaOH were used as chromatgraphy media and chromatography solvent respectively. After chromatography, radiolabeled nucleic acid probes stayed at origin, while free nucleotides mi- grated to solvent front. Thus the radioactivity of radiolabeled nucleic acid and nucleotide could be measured respectively. This method is very simple, and could be finished in 30 min. On the other hand, this method leads to less contamination for the only radioactive waste was nylon filter itself after detection.

关 键 词： 核酸探针 放射性同位素 快速检测 核酸杂交 尼龙膜

领　　域： [生物学]