作　　者： ; ; ; ; ; ; ;

机构地区： 重庆大学生物医学工程联合学院生物流变科学与技术教育部重点实验室

出　　处： 《生物物理学报》 2004年第3期167-172,共6页

摘　　要： 探讨了integrinβ1在不同细胞周期人肝癌细胞(SMMC-7721)上的表达和在肝癌细胞与人脐静脉内皮细胞粘附过程中的作用。未同步处理的肝癌细胞(对照组)各细胞周期时相百分比为G0/G1期53.51%、G2/M期11.01%、S期35.48%,采用胸腺嘧啶脱氧核苷、秋水仙碱顺序阻断和胸腺嘧啶脱氧核苷双阻断后释放培养的方法获得G1期和S期的肝癌细胞,其同步率分别为74.09%和98.29%。G1期肝癌细胞integrinβ1表达的荧光强度较S期和对照组相应值明显降低。利用微管吸吮技术定量研究了肝癌细胞与内皮细胞之间的粘附力学特性,发现G1期肝癌细胞的粘附力值比S期相应值明显降低(P<0.01),而S期的粘附力值与对照组比较无明显差别,integrinβ1在肝癌细胞与内皮细胞粘附过程中的贡献约50%。结果提示:胸腺嘧啶脱氧核苷和秋水仙碱能较好地将肝癌细胞同步于G1期和S期,integrinβ1在SMMC-7721肝癌细胞上的表达水平呈现周期差异,在肝癌细胞与内皮细胞粘附过程中,S期细胞可能起的作用更大,integrinβ1在这一粘附过程中起着重要作用。 To investigate expression of integrin yclic hepatocellular carcinoma cells (HCC) and human umbilical vein endothelial cells (HUVECs), the synchronous G1 and S phase HCC were achieved through thymine-2-desoryriboside and colchicines sequential blockage method and double thymine-2-desoryriboside blockage method, respectively. Expression of integrin ve course were studied by micropipette aspiration technique. The results showed that percentage of each phases of the control HCC cell cycle are 53.51% of G0/G1 phase?11.01% of G2/M phase and 35.48% of S phase, while the synchronous rates of G1 and S phase SMMC-7721 cells amount to 74.09% and 98.29%, respectively. The expressive fluorescent intensity of integrin to HUVEC was significantly lower than the value of S phase cell(P<0.01), but it has no remarkable difference when compared the value of S phase HCC with controlled HCC; the contribution of integrin C. It suggested that HCC would be synchronized preferably in G1 and S phase with thymine-2-desoryriboside and colchicines, the expression of adhesive molecule integrin in HCC's adhesion to HUVEC, and integrin

关 键 词： 肝癌细胞 细胞周期 粘附 微管吸吮技术 内皮细胞

领　　域： [生物学] [生物学]