作　　者： ; ; ; ;

机构地区： 湖南农业大学

出　　处： 《微生物学报》 2004年第3期406-410,共5页

摘　　要： 基因芯片因其具有高密度、高灵敏度、快速 (实时 )检测、经济、自动化和低背景水平等特点 ,而广泛应用于不同的研究领域。目前 ,应用于环境微生物研究的基因芯片主要有功能基因芯片 (FGAs)、系统发育的寡核苷酸芯片 (POAs)和群落基因组芯片 (CGAs)。综述了基因芯片在环境微生物研究中的应用 ,包括自然环境中微生物的基因表达分析、比较基因组分析和混合微生物群落的分析等。 Microarray take advantage of the advanced technologies of molecular biology, semi-conduct microelectronics, laser, chemical dyes and so on, and is the high-cross and comprehensive advanced science and hot spot of study. Compared with traditional nucleic acid methods, microarray offer the additional advantages of high density, high sensitivity, rapid (real-time) detection, lower cost, automation, and low background levels and so on. Therefore, microarray-based technology has been used in many fields and is potentially well suited for identifying populations of microorganisms in natural environments. The microarray used in environmental microbial studies can be divided into three major classes, including functional gene arrays(FGAs), phylogenetic oligonucleotide arrays(POAs) and community genome arrays(CGAs). In this review, we viewed microarray's application to research on environmental microorganisms, such as analyzing microbial gene express in environment, comparable genome analysis and environmental microbial community analysis. At last, the challenges and future perspectives of microarray in natural environment was discussed.

关 键 词： 基因芯片 环境微生物 群落结构 功能基因

领　　域： [生物学]