作　　者： ; ; ; ;

机构地区： 中山大学生命科学学院基因工程教育部重点实验室

出　　处： 《菌物学报》 2004年第2期255-261,共7页

摘　　要： 本文首次建立了一种通过PEG转化缓冲液将外源基因转入灵芝的方法,转化频率约为5～6个/μg(抗性转化子/质粒+107个原生质体).转化子在不含HmB的培养基上经5代以上的继代培养后仍可以稳定表达HmB抗性.Southern blot检测证明外源基因已经整合到了灵芝的基因组DNA中.本研究为通过基因工程手段定向、快速改良灵芝药用品质以及利用灵芝发酵方法生产一些具有重大经济价值的外源蛋白等应用奠定基础,并且也有助于我们进一步了解灵芝这一大型真菌中的基因的表达调控机制. A procedure of transforming foreign genes into Ganoderma lucidum using PEG buffer has been established. The transformation efficiency of this method reached to 5-6 transformants/mg plasmid+107 protoplast.Transformants could still show HmB resistance after cultivating over 5 subcultures on media without HmB. An analysis of Ganoderma lucidum transformants using Southern blot revealed that foreign gene had integrated into Ganoderma lucidums genome.This study has laid a foundation for those applications, such as improving medical quality of Ganoderma lucidum by gene engineering and producing foreign protein with important economic value by fermentation of Ganoderma lucidum.It is also helpful for us to understand mechanism of gene expresstion and regulation about Ganoderma lucidum.

关 键 词： 法 灵芝 药用菌 转化效率 外源基因

领　　域： [生物学] [生物学]