作　　者： ; ; ; ; ;

机构地区： 暨南大学生命科学技术学院水生生物研究所

出　　处： 《生态科学》 2004年第1期5-8,共4页

摘　　要： 采用海洋微藻四列藻为试验材料,试验前期微藻经光限制(500 LX)胁迫处理10 d,试验后期重新接种并恢复正常光照(5000 Lx)培养10 d,均以正常光照(5 000 Lx)培养作对照,测定四列藻的细胞密度、细胞内蛋白质含量、细胞内糖分含量等指标。结果表明,处理组在恢复工常光照的初期(1～2 d)细胞生长量超过对照(P<0.05),即藻细胞平均相对生长率提高,细胞数增多,叶绿素 a含量和生物量增加,细胞数净增率最高达31.6％,生物量净增率最高达34.8％。同时,四列藻在受到光限制胁迫后,藻细胞内蛋白质、细胞内糖和蛋白质/糖(P/C)的比值发生了变化,但是在恢复正常光照培养后,这几个指标均逐渐恢复至处理前的水平。四列藻的这种受到光胁迫后细胞生长量增加的特征表明微藻具有超补偿性能。 Micro-alga Tetraselmis tetrathele was used as experimental material. In the initial time of experiment, T tetrathele had been treated under a stress of light limitation (500 Lx) for ten days, while in the later time, it had been cultured for ten days after re-inoculated and recovered normal light (5 000 Lx), and the normal light (5 000 Lx) culture was used as all the controls. In the course of the experiment, density of cells, content of intracellular protein, content of intracellular carbohydrate and other indexes were determined. The result showed that the treated group had a more amount of cell growth than the control group (P<0.05) in the initial time (1～2 d) after light recovery,that is, the treated group had a higher average relative growth rate, a more number of cells, a more biomass and a higher chlorophy11-a content than those of the control group, in addition, the maximal rate of net cells increase was 31.6%, and the maximal rate of net biomass increase was 34.8%. Meanwhile, the intracellular protein, the intracellular carbohydrate and the ratio of protein to carbohydrate (P/C) changed under the stress of light limitation, but all of them would return to their initial levels in the following culture after light recovery. The character of T. tetrathele had a more amount of cell growth showed that the micro-algae had an overcompensatory ability.

关 键 词： 四列藻 光胁迫 超补偿生长 生态学 细胞生长量

领　　域： [生物学] [生物学]