机构地区: 河北大学生命科学学院
出 处: 《海洋科学》 2004年第5期58-62,共5页
摘 要: 以患病死亡的甲壳上具白斑的斑节对虾(Penaeusmonodon)虾头及从中提取的病毒悬液分别感染中国对虾(Penaeuschinensis)幼虾及日本对虾(Penaeusjaponicus)。感染后此2种对虾均发病死亡 ,且甲壳上均有白斑。电镜下对人工感染的中国对虾幼虾及日本对虾的组织器官进行观察发现了大量的病毒。此病毒为杆状 ,具囊膜 ,无包涵体 ,大小为295nm×105nm。此病毒的形态发生起始于细胞核 ,并可分为4个阶段 :病毒基质的形成、囊膜的形成、病毒的装配和完整的病毒粒子的形成。病毒的装配过程是与其大量迅速繁殖相适应的。 Larvae of Penaeus chinensis were fed with the heads of Penaeus monodon affected with white spots on the carapace while Penaeus japonicus were injected with virus suspension also from Penaeus monodon affected with white spots on the carapace.After infection,larvae of P.chinensis and P.japonicus all died,white spots were found on their caraˉpaces.A non-occluded baculovirus was observed from the artificially infected larvae of Penaeus chinensis and Penaeus japonicus.The virion was enveloped with a mean size of295nm×105nm.Beginning in the nuclei the viral morphogenesis process was composed of four stages:formation of viral stroma,formation of membrane,assembly of the virus and formation of complete virons.The assembling procedure allows large and rapid propagation of the baculovirus.