作　　者： ; ; ;

机构地区： 华南农业大学动物科学学院

出　　处： 《华南农业大学学报》 2004年第2期89-92,共4页

摘　　要： 应用RT PCR和nPCR实现了口蹄疫病毒O/PanAsia/10株的全衣壳基因的克隆和序列测定.对核苷酸和氨基酸的序列分析表明,与经典O型毒株比较存在较大变异.其中4种结构蛋白的变异顺序是VP1>VP3>VP2>VP4.P1基因G+C值约55%.全衣壳蛋白相对分子质量约8 0×104.主要结构蛋白VP1呈碱性,等电点约9 5. A reverse transcription polymerase chain reaction (RT-PCR) and nested PCR (nPCR) is described that amplified the genes encoding the capsid proteins VP1-4 of the foot-and-mouth disease (FMD) virus (O/PanAsia/10.) The sequences of the antigenically relevant capsid proteins VP1-3 were compared with those of previous field viruses and found to be highly variable by phylogenetic analysis as following:VP1>VP3>VP2. The G+C value of P1 gene is about 55% and the size of the whole capsid protein is about 8.0×10~4. The major structural protein VP1 is basic and its isolectric point is pH 9.5, much higher than those of VP2, VP3 or VP4.

关 键 词： 口蹄疫病毒 泛亚株 衣壳蛋白 基因克隆 序列分析 口蹄疫

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]