作　　者： ; ; ; ;

机构地区： 沈阳农业大学

出　　处： 《辽宁农业科学》 2004年第2期1-4,共4页

摘　　要： 在PEG渗透胁迫下,6个高耐旱性大豆品种幼苗叶片中游离脯氨酸含量升高至274.9～441.2μg/g干重,内源脱落酸含量升高至18.66～28.83μg/g干重;4个高敏感型大豆品种游离脯氨酸含量为145.6～258.9μg/g干重,内源脱落酸含量为8.79～16.62μg/g干重。从中选出高耐旱(T5)和高敏感(S2)的大豆各一品种。两品种幼苗叶和根在4%PEG胁迫处理0～96h期间,AsA POD、CAT酶活性,以及TTC还原量变化趋势均为"先下降再上升,然后再次下降,再次上升";MDA含量的变化趋势与前三者恰好相反。而SOD酶活性在胁迫初期(0～36h)首先升高,36h的变化趋势与POD和CAT酶变化趋势一致。逐步回归定量分析结果指出,AsA POD酶活性是影响大豆叶细胞生存力和耐旱能力的关键酶。 Under 6% PEG treatment, the content of free proline and endogenous abscisic acid(ABA) in young leaves and roots of 6 drought-tolerant soybean cultivars increased up to the range of 274.9～441.2 μg/g DW and 18.66～28.83 μg/gDW, respectively. The content of free proline and ABA of 4 drought-sensitive cultivars was in the range of 145.6～258.9 μg/gDW and 8.7916.62 μg/gDW, respectively. Based on the above data, each of the drought-tolerant and sensitive cultivars was selected for further analysis. Under 4% PEG treatment, AsA-POD, CAT activities and TTC reduction changed with the treatment time (h), showing a tendency of 'to decline and increase alternately'twice. MDA content changed contrarily to the former tendency. SOD activities increased rapidly at first with POD and CAT activity. Stepwise regression equation of TTC reduction of different soybean cultivars showed that AsA-POD is the key enzyme in determining the ability of drought tolerance in soybean leaves.

关 键 词： 大豆 耐旱性 抗氧化保护酶 活性 渗透胁迫 逐步回归分析

领　　域： [农业科学]