机构地区: 中国科学院海洋研究所
出 处: 《海洋科学》 2004年第4期8-12,共5页
摘 要: 通过提取健康养殖牙鲆(Paralichthys olivaceus)血清中的免疫球蛋白(Ig),并测定其相对分子质量,其中重链(H链)的相对分子质量为74.2 ku,轻链(L链)的相对分子质量为23.8 ku。用纯化的牙鲆血清中的Ig作抗原,制备多克隆抗体,确立牙鲆血清中免疫球蛋白的最佳测定方法。 The serum IgM of healthy cultured flounder was purified and the molecule of the IgM was measured. The height chain (H chain) molecule weight and the light chain (L chain) molecule weight of the purified serum IgM were 74.2 ku and 23.8 ku. The polyclonal antibodies anti-serum IgM of flounder can be obtained by injecting the animals with the purified IgM . This study provided a rellable method of measuring the concentration of the serum IgM in fish.