作　　者： ; ; ; ; ; ; ;

机构地区： 中国水产科学研究院珠江水产研究所

出　　处： 《上海水产大学学报》 2004年第1期23-29,共7页

摘　　要： 从患溃疡病的鳜肾脏中分离出一株病原细菌编号为GYK1。此株病菌人工感染鳜、银鲫、剑尾鱼,实验鱼出现出血、肌肉坏死或溃疡症状;腹腔注射攻毒,对鳜、银鲫、剑尾鱼、小鼠的半致死剂量(LD50)分别为8.33×104、1.06×105、1.26×105、1.05×106CFU/g;菌培养液上清能溶解鳜、加洲鲈、银鲫、小鼠、兔、绵羊、人O型血等的红细胞,在绵羊血平板上为β 溶血,不同代次和保存条件的细菌溶血性稳定。细菌在电镜下的形态、生化特性和ID32E系统自动鉴定的结果均符合嗜水气单胞菌的特征;PCR扩增检测到嗜水气单胞菌特异性的毒力基因气溶素基因(aerA)209bp片段,进一步说明此株病原细菌为含有毒力基因的嗜水气单胞菌。实验结果表明,GYK1是一株毒力和溶血性均较强的嗜水气单胞菌。 A pathogenic bacterial strain named GYK1 was isolated from the kidney of mandarinfish (Siniperca chuatsi Basilewsky) suffering from epizootic ulcerative syndrome. The virulent tests were carried out by using the pure culture of the bacteria artificially infected Siniperca chuatsi, Carassius gibelio, Xiphophorus helleri and mouse. The experimental fish died of hemorrhage, necrosis or ulcer in the muscles. The LD(50) of the bacteria infecting the three species of experimental fish and the mouse via intraperitoneal injection was 8.33×10~4,1.06×10~5,1.26×10~5 and 1.05×10~6 CFU/g respectively. The supernatant of the culture could lyse erythrocytes not only from mandarinfish but also from large-mouth bass, silver crucian carp, mouse, rabbit, sheep, and the O-serotype human. The bacteria had β-hemolytic activity in 5% sheep blood agar. And the hemolytic activity was stabilized in different generations or conserved conditions. The isolate was defined as A.hydrophila based on the morphology in TEM, biochemical characteristics and the automatic ATB Expression. The specific 209bp fraction in the aerolysin gene of A.hydrophila was amplified by the polymerase chain reaction. It proved that the isolate was A. hydrophila with the aerolysin gene whose product having both hemolytic and cytolytic properties. The tests showed GYK1 was a strain of A. hydrophila with strong virulence and hemolytic activity.

关 键 词： 鳜 嗜水气单胞菌 鉴定 毒力 溶血性

领　　域： [农业科学]