作　　者： ; ; ;

机构地区： 中国科学院海洋研究所

出　　处： 《生物化学与生物物理进展》 2004年第3期273-277,共5页

摘　　要： 从单细胞蓝藻钝顶螺旋藻中纯化C 藻蓝蛋白 ,从海洋红藻多管藻纯化R 藻红蛋白 .分别用高碘酸钠氧化法和戊二醛法将二者共价连接为R 藻红蛋白 C 藻蓝蛋白交联物 ,再用SephadexG 2 0 0柱层析纯化 .光谱分析表明 ,用两种方法构建的共价交联物都可以将激发能从R 藻红蛋白传递到C 藻蓝蛋白 .二者相比 ,高碘酸钠氧化法构建的共价交联物的能量传递效率更高 . The C-phycocyanin and the R-phycoerythrin were purified from the blue-green alga Spirulina platensis and red alga Polysiphonia urceolata respectively. Both sodium periodate and glutaraldehyde are effective coupling agents being capable of constructing the R-phycoerythrin-C-phycocyanin conjugate, which was also called phycobiliproteins energy transfer model. The two artificial conjugates constructed with different methods were purified by Sephadex G-200 chromatography respectively. Spectra analysis indicated that energy transfer occurred in the two conjugates. The conjugate with sodium periodate had the higher efficiency of energy transfer than that with glutaraldehyde conjugate.

关 键 词： 藻红蛋白 藻蓝蛋白 高碘酸钠氧化交联法 戊二醛交联法 共价交联物 光谱分析 传能效率

领　　域： [生物学]