作　　者： ; ; ; ; ;

机构地区： 中山大学中山医学院

出　　处： 《遗传》 2003年第3期276-278,共3页

摘　　要： 为了探讨具有不同放射敏感性的CNE1、CNE2鼻咽癌细胞株中ATM/PI3K区基因突变的情况,采用反转录聚合酶链式反应(RT-PCR)技术和PCR产物直接测序方法(荧光标记的ddNTPs循环测序),对CNE1、CNE2中ATM的PI3K关键区域进行突变检测。结果表明,所测CNE1、CNE2中的ATM/PI3K区序列中没有发生突变,认为鼻咽癌细胞株CNE1、CNE2间内在的放射敏感性的差异可能与ATM/PI3K区基因突变不相关。 To try to elucidate the relationship between the radiosensitivity of NPC cell lines and ATM gene,this study was designed to investigate the mutation of ATM/PI3K region in NPC cell lines with different radiosensitivity.Two NPC cell lines of CNE1 and CNE2 with different radiosensitivities were established.Reverse transcriptase polymerase chain reaction (RT-PCR) was used to get a 546bp fragment(8578nt～9123nt) of ATM cDNA,containing the PI-3 kinase domain (8753～8815bp).Direct sequencing of RT-PCR product was applied to determine the mutations in the gene.The sequence of the 546bp fragment is identical to the sequence of ATM gene published in GenBank,that is,there are no any mutations on the fragment of ATM/PI3K we examined either in CNE1 or in CNE2.It indicates that there may be no correlations between the mutation of ATM/PI3K and the variation of radiosensitivity in NPC cell lines CNE1,CNE2.

关 键 词： 鼻咽癌 细胞株 区 基因突变 放射敏感性

领　　域： [生物学] [生物学]