作　　者： ; ; (夏枫耿）; (董加喜）;

机构地区： 华南理工大学生物科学与工程学院生物工程系

出　　处： 《昆虫天敌》 2004年第1期1-6,共6页

摘　　要： 金龟子绿僵菌属于真菌类生物杀虫剂,本文研究了接种量、种龄、菌种代数等生物因素对金龟子绿缰菌生长及产孢量的影响,并对液—固两步发酵工艺进行了研究。经过优化,得到金龟子绿僵菌产孢的最佳生物参数是:接种量为2.5g/100g、种龄为5-6天;采用固体二代种,培养6天,孢子产量可达1.531×10^(10)孢子/g干培养基。 Metarhizium anisopliae is a kind of fungal insecticide. The effects of biological variables, such as inoculation ratio, seed age and seed generation etc. , on growth and sporification of Metarhizium anisopliae in solid - state fermentation were investigated. The technology of two - stage fermentation was also studied. The results showed that optimal biological conditions of solid - state fermentation of Metarhizium anisopliae were as following: inoculation ratio 2.5g/100g and seed age 6 days. The maximum spore yield reached to 1.531×1010/g dry medium in 6 day culture by inoculating the second generation seed.

关 键 词： 金龟子绿僵菌 固态发酵 生物参数 固态培养

领　　域： [农业科学] [农业科学]