作　　者： ; ; ; ;

机构地区： 汕头大学理学院生物系

出　　处： 《食品科学》 2004年第3期159-162,共4页

摘　　要： 龙须菜藻体经细胞破碎,其水溶性提取物经硫酸铵沉淀即得藻胆蛋白粗提物。该粗提物经Sephadex G-100柱层析,可分离出藻红蛋白(PE),其在聚丙烯酰胺凝胶电泳中仅显示一条带,用SDS-PAGE不连续电泳测定其亚基分子量分别为18000Da和24000Da。该PE的吸收光谱有二峰一肩,最大吸收值在570nm处,属I型R-PE。分别采用Fenton体系和邻苯三酚-鲁米诺发光体系测定藻胆蛋白粗提物及分离的PE清除·OH和O2·能力,结果表明,藻胆蛋白粗提物和分离的PE均有清除自由基作用,但两者间存在差异。 The crude phycobiliproteins were got from water extraction of smashed cells of Gracilaria lemaneiformis wereprecipitated in ammonium sulfate. Phycoerythrin could be separated through chromatographed the crude phycobiliproteins bySephadex G-100 column, and it appeared only one band in the polyacrlamide gel electrophoresis. Molecular weights of subunitsof the phycoerythrin are 18000Da and 24000Da that were measured by SDS-PAGE. The PE possessed an absorption maxi-mum at 570nm and it belongs to R-PE type Ⅰ.The scavenging activities of the crude phycobiliproteins and the PE on OH ofFenton reaction and O2· f Luminol reaction were studied.The results showed that the crude phycobiliproteins and the PE bothcould scavenge free radicals, but their abilities were different.

关 键 词： 龙须菜 藻胆蛋白 分离 自由基 清除作用 吸收光谱

领　　域： [轻工技术与工程] [轻工技术与工程]