机构地区: 华中农业大学畜牧兽医学院
出 处: 《中国兽医学报》 2004年第2期145-147,共3页
摘 要: 用含有日本乙型脑炎病毒 (SA14 - 14 - 2株 )非结构蛋白 NS1基因的重组伪狂犬病病毒 TK- / g G- / NS+ 1 免疫BAL B/ c小鼠和断奶仔猪。结果表明 ,该重组病毒对 BAL B/ c小鼠和断奶仔猪是安全的 ,免疫的 BAL B/ c小鼠能抵抗伪狂犬病病毒 (PRV )强毒 (Ea株 )的致死性攻击 ,免疫的断奶仔猪能产生乙型脑炎病毒 (JEV)特异性抗体和 JEV特异性 CTL 活性。 Mice and piglets were immunized with a recombinant pseudorabies virus TK^-/gG^-/NS^+_1 containing NS_1 gene of Japanese encephalitis virus(JEV).The results illustrated that the recombinant vaccine was safe for mice and piglets,immunization with the recombinant vaccine was sufficient to protect mice against a lethal PRV Ea strain challenge,and immunization of piglets with TK^-/gG^-/NS^+_1 vaccine could produce specific antibody against JEV and JEV-specific CTL activity.