作　　者： ; ; ; ; ;

机构地区： 中山大学生命科学学院有害生物控制与资源利用国家重点实验室

出　　处： 《中国环境科学》 2004年第1期9-13,共5页

摘　　要： 在Hoagland培养基中加入CuSO4模拟Cu2+污染环境,比较了不同Cu2+浓度([Cu2+])对鸭跖草(Commelina communis L.)耐性生态型和敏感生态型的耐性、Cu积累和抗氧化酶[过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和过氧化物酶(POD)]的影响.结果表明,两种生态型鸭跖草的Cu2+中毒临界浓度和中毒症状有明显的差异,[Cu2+]≥40祄ol/L时,耐性生态型鸭跖草的耐性明显高于敏感生态型.耐性生态型鸭跖草叶和根中的Cu2+含量都明显高于敏感生态型,根中更为明显.不同生态型甚至同一生态型不同器官的POD、CAT、SOD活性对Cu2+的敏感性不同.一般来说,根对Cu2+的敏感性高于叶;POD活性对Cu2+的敏感性高于CAT和SOD.根比叶在清除丙二醛(MDA)的过程中起更重要作用.耐性生态型鸭跖草叶和根中CAT、POD和SOD活性、叶中MDA的变化并没有与根中MDA含量的变化趋势(大幅增加)保持一致,表明除了抗氧化酶外,可能还存在其他机制影响耐性生态型与敏感生态型鸭跖草在Cu2+耐性上的差异. CuSO4 was added into Hoagland cultivation base to imitate Cu2+ polluted environment, and the effect of different Cu2+ concentration on tolerance, Cu2+ accumulation, and antioxidative enzymes (CAT, SOD, and POD) of Commelina communis ecotype (tolerant ecotype and sensitive ecotype) were compared. The critical concentration and poisoning symptom of two ecotypes of C. Communis were different markedly. At [Cu2+]≥40祄ol/L, tolerance of tolerant ecotype is higher significantly than that of sensitive ecotype, and Cu2+ concentration of tolerant ecotype in leaves and roots were higher significantly than that of sensitive ecotype, especially in roots. It was different from sensitivity of CAT, SOD, and POD of different ecotype, even of different organ of same ecotype activity on Cu2+. Generally speaking, sensitivity of roots on Cu2+ concentrations was higher than that of leaves, and that of POD activity was higher than that of CAT and SOD activity. Roots played more important action than leaves in cleaning up MDA. Unlike sensitive ecotype, CAT, SOD, and POD activity in leaves and roots and MDA change in leaves did not agree with the change trend (increasing vastly) of MDA content in roots of tolerant ecotype which indicated that there existed other mechanism influencing the difference from the tolerance of two ecotype of C. Communis on Cu2+ except the antioxidative emzymes.

关 键 词： 生态型 抗氧化酶 鸭跖草

领　　域： [环境科学与工程]