作　　者： ; ; ; ; ;

机构地区： 南京农业大学资源与环境科学学院

出　　处： 《生态学杂志》 2004年第1期30-33,共4页

摘　　要： 采用透析、凝胶色谱 (SephadexG 75 )研究了污泥堆肥前后腐殖质分子大小的变化及重金属Cu和Zn在各级组分中的分配。透析结果表明 ,污泥经过堆腐以后 ,腐殖质中小分子物质 (<10 0 0Da)组分的含量下降 6 4 % ,而相对高分子组分 (>2 5 0 0 0Da)却增加了 6 8%。凝胶色谱进一步证实 ,污泥经过 4 9d堆腐后 ,腐殖质中大于 2 0 0 0KDa的大分子组分是堆肥起始时的2 3倍。而小分子组分明显减少 ,表现在小分子组分的凝胶洗脱体积明显减少。堆肥腐熟以后 ,腐殖质吸附的Cu、Zn元素含量增加 ,其中Cu主要被吸附在大分子物质上 。 Molecular size distribution of humic substance before and after sludge composting was investigated by dialysis and Sephadex Gel-75 chromatography. Cu and Zn in each fractions of different molecular size were also determined. The results showed that after composting, the content of low molecular size fraction with molecular weight cut off <1000 Da in humic substance decreased by 64% while high molecular size fraction with >25?000 Da increased by 68%. The evidence from the Gel-75 chromatography also further confirmed that the content of the higher molecular size fraction with >2?000 KDa in composted sludge was 2.3 times higher than that of non-composted sludge. In the different fractions of humic substance, Cu was found to be more favorably bound with high molecular size fraction, but Zn was easily associated with low molecular size fraction.

关 键 词： 污泥堆肥 腐殖质 重金属

领　　域： [农业科学] [农业科学]