作　　者： ; ; ; (卜淑敏）;

机构地区： 中国农业大学生物学院

出　　处： 《农业生物技术学报》 2003年第3期291-294,共4页

摘　　要： 采用腹腔单独注射L-NAME(左旋硝基精氨酸甲基酯)或联合注射L-NAME和L-Arg,取出卵母细胞体外观察,研究了NO对小鼠卵母细胞体内成熟的影响。结果表明,(1)单独注射5、10、20mg/kg体重L-NAME均能显著抑制卵母细胞第一极体的释放(P<0.01),尤以10mg/kg体重L-NAME组作用最为明显,各剂量组对生发泡的破裂均无影响(P>0.05);(2)10mg/kg体重L-NAME对卵母细胞成熟的抑制作用能被同时注射的100mg/kg或200mg/kg体重L-Arg逆转,其中以200mg/kg体重作用最为明显;(3)L-NAME对卵母细胞畸形率的影响和培养时间有关,而对卵母细胞的存活率没有统计学影响。实验结果证明NO参与了小鼠卵母细胞的体内成熟调控。 The effect of nitric oxide (NO)on meiotic maturation of mouse oocyte in vivo was investigated by injecting L-NAME alone or co-injecting L-NAME and L-Arg intraperitoneally. The results showed that: (1) 5, 10 and 20 mg/kg bw (body weight) of L-NAME inhibited significantly the extrusion of first polar body of mouse oocyte(P <0.01), especially 10 mg/kg bw. But they all had little impact on germinal vesicle breakdown (P >0.05). (2) The inhibitory effect of L-NAME (10 mg/kg bw) on the extrusion of first polar body of mouse oocyte could be reversed by 100 mg/kg bw or 200 mg/kg bw L-Arg.(3) The abnormality caused by L-NAME was related to the culture time, but L-NAME had no significant effect on viability of mouse oocytes. It could be concluded that NO was involved in regulation of mouse oocyte meiotic maturation in vivo.

关 键 词： 卵母细胞 减数分裂 合酶 小鼠 一氧化氮 卵巢

领　　域： [生物学]