作　　者： ; ; ; (钟前林）;

机构地区： 华南农业大学食品学院

出　　处： 《微生物学杂志》 2004年第1期5-7,共3页

摘　　要： 经筛选和诱变 ,选育出 1株产 β 木聚糖酶活力较高 ( 1 2 4.45IU/mL) ,而羧甲基纤维素酶活力较低( 1 .0 8IU/mL)的突变株L1 0 IGA。得出该菌产酶的最适发酵工艺为 1 .5 %半纤维素 ,0 .1 2 7%蛋白胨 ,0 .1 0 5 %K2 HPO4,0 .0 5 5 %MgSO4·7H2 O ,0 .45 %酵母膏 ,0 .2 2 5 %Twen40 ,装量 3 2 .5mL/1 5 0mL三角瓶 ,起始pH为 7.2 5 ,3 7℃发酵 48h后 45℃发酵 2 4h。 A stable β-xylanase producer-mutant L10-IGA was filtrate out through treatment with UV,NTG,UV on wild strain L-10. The β-xylanase and CMCese activities of L10-IGA were 124.45 IU/mL,and 1.08 IU/mL, respectively.The optimal medium were consisted of 1.5% hemicelluse,0.45% yeast extract,0.127% peptone,0.105% K-2HPO-4, 0.055% {MgSO-4}, 0.225% Twen80. The optimal culture conditions are following:initial pH was 7.25,temperature was 37℃ 48h and 45℃ 24h,culture medium cubage was 32.5mL/150mL burgee.

关 键 词： 木聚糖酶 选育 发酵工艺 高产菌

领　　域： [轻工技术与工程] [化学工程]