作　　者： ; ; ; (李兰会）;

机构地区： 河北科技师范学院

出　　处： 《畜牧兽医学报》 2015年第11期1934-1943,共10页

摘　　要： 本研究旨在探讨白色和黑色山羊皮肤组织Agouti基因所具有的不同剪接体类型。采用PCR扩增和5′RACE方法分别获得白色和黑色山羊皮肤组织Agouti基因部分基因组序列及mRNA序列,并进行拼接比对。结果,分别获得了长度为27 793和27 858bp的白色和黑色山羊Agouti基因序列,发现4段序列(88、180、81和79bp)与绵羊mRNA序列相匹配,这些片段与5′RACE所检测到的外显子1可变剪接体长度对应,其中的180、81和79bp为新发现片段。白色山羊检测到It、It′、IA、2C和IE 5种类型剪接体,黑色山羊仅检测到It和It′2种类型剪接体。剪接体之间和剪接体内部均存在长度变异。结果提示,IA、2C和IE可变剪接可能与山羊毛色形成有关。 The objective of this study was to investigate the variant types of goat Agouti gene spliceosome in goat skin with different coat color.The sequences and mRNA of goat Agouti gene were obtained and analyzed from goat skin with different coat color by PCR and 5′RACE methods,respectively.The 27 793 and 27 858 bp of partial Agouti gene sequences were obtained from white and black goat skin,respectively.Four fragments,88,180,81 and 79bp,in which the 180,81 and 79bp were first detected in goat,were matched with the sequences of sheep mRNA and corresponded with the length of exon 1spliceosome obtained by 5′RACE.The 5types(It,It′,IA,2C and IE)of spliceosome were detected in goat skin with white coat color,and only 2types(It and It′)were found in goat skin with black coat color.There was length variation within and among spliceosomes.The result indicate that the IA,2Cand IE might be related with the formation of goat coat color.

关 键 词： 山羊 基因 皮肤组织 剪接体类型

领　　域： [农业科学] [农业科学]