作　　者： ; ; ; ; ; ; ;

机构地区： 中国农业科学院蜜蜂研究所农业部授粉昆虫生物学重点开放实验室

出　　处： 《畜牧兽医学报》 2015年第9期1638-1643,共6页

摘　　要： 为了探明中国主要养蜂地区蜜蜂微孢子虫的种类及分布,采用多重PCR的方法对采自中国部分主要养蜂地区的68份蜜蜂样品中含有的微孢子虫进行种类鉴定。结果发现所有样本中仅采自山东寿光的样品中检测出蜜蜂微孢子虫(Nosema apis),其他所有样品均只检测出东方蜜蜂微孢子虫(Nosema ceranae)的存在。初步推断在中国主要养蜂地区寄生危害西方蜜蜂的微孢子虫种类主要是东方蜜蜂微孢子虫,该结果为微孢子虫病在中国的流行规律及防治研究奠定了一定的基础。 In order to clarify distribution of Nosemagermplasm in China,68 samples of adult honey bee from 16 provinces of China were collected.All of the samples which with clear signs of population depletion and positive to microsporidian spores using light microscopy were selected for molecular germplasm identification by method of multiplex PCR.The sequenced products of all of the 68 samples were identical to the corresponding Nosema ceranae sequence.Only one of samples collected from Shandong was found that had infected by Nosema apis.The results indicated that vast majority species of nosema infect Apis melliferain China is Nosema ceranae,Nosema apis is very rare within Apis mellifera.

关 键 词： 西方蜜蜂 微孢子虫 多重 种群分布

领　　域： [农业科学] [农业科学]