作　　者： ; ; ; ;

机构地区： 华南农业大学动物科学学院

出　　处： 《畜牧兽医学报》 2015年第7期1157-1162,共6页

摘　　要： 本研究旨在对转GDF9shRNA基因小鼠进行扩群繁殖,统计分析转基因小鼠的产仔数及增重,检测GDF9shRNA、GDF9表达水平以及血清中FSH和GH的水平,以观察GDF9的下调对转基因小鼠繁殖性能及生长发育的影响。通过RT-PCR方法,分别检测了GDF9shRNA在体内的表达,以及下丘脑、垂体和卵巢中GDF9mRNA表达水平;同时用ELISA检测了血液中FSH和GH水平。结果表明,扩群后得到16只GDF9shRNA阳性小鼠,转基因GDF9shRNA在小鼠体内广泛表达,转基因小鼠的产仔数与野生型小鼠相比差异显著(P<0.05);转GDF9shRNA阳性公鼠的体重比阴性公鼠高,且在第7周差异显著(P<0.05),而母鼠间体重无显著差异;与阴性小鼠相比,转GDF9shRNA阳性小鼠GDF9基因的表达量在下丘脑、垂体中分别下降35.4%和39.8%,差异显著(P<0.05),在母鼠卵巢中表达水平差异不显著(P>0.05);阳性母鼠血液中FSH水平比阴性母鼠高55%,差异极显著(P<0.01)。结果表明,GDF9基因下调可以提高动物繁殖性能,为提高大型牲畜繁殖力提供了有利的素材和靶点参考。 This experiment aims to detect the effect of down-regulation of GDF9 on reproduction and growth in GDF9 shRNA transgenic mice through statistical analysis of the litter size and weight gain of transgenic mice and detection of biomolecular and blood biochemical indexes such as GDF9 shRNA,GDF9and serum FSH and GH level after reproduction.The levels of GDF9 shRNA and GDF9 mRNA expression were detected in hypothalamus,pituitary and ovary via RT-PCR,and serum FSH and GH level were examined using ELISA.The results showed that 16GDF9 shRNA transgenic mice obtained were positive,and GDF9 shRNA in transgenic mice was widely expressed in various tissues.Litter sizes between transgenic and wild mice were significantly different(P<0.05);Body weight of transgenic male mice was higher than that of control mice with significant difference(P<0.05)in the seventh week,and body weight of female mice did not show significant difference.Compared with the negative mouse,GDF9 gene expression in transgenic mice in hypothalamus and pituitary were decreased by 35.4% and 39.8%(P<0.05),respectively.In transgenic female mice,GDF9 expression in ovaries was not significantly decreased(P>0.05),but its serum FSH level was 55% higher than the control(P<0.01).The present study showed down-regulation expression of GDF9 gene can improve the ability of animal reproduction.It may provide a favorable material and molecular target for producing transgeniclarge livestock with high reproduction.

关 键 词： 干涉 繁殖 转基因小鼠

领　　域： [农业科学] [农业科学]