作　　者： ; ; ; ; ;

机构地区： 河北农业大学动物科技学院

出　　处： 《畜牧兽医学报》 2014年第12期1932-1938,共7页

摘　　要： 旨在探讨Agouti基因调控山羊毛色机制,采用SYBR Green real-time PCR和蛋白免疫印迹方法分别检测了不同毛色山羊皮肤组织中Agouti基因mRNA及其所编码ASIP蛋白的差异表达。结果表明,Agouti基因mRNA在白色、黑色、棕色山羊皮肤组织中的表达量依次为白色>黑色>棕色;mRNA表达量在棕色和白色、棕色和黑色山羊皮肤之间均存在显著差异(P<0.05),在白色和黑色山羊皮肤之间差异不显著(P>0.05)。ASIP在白色、黑色、棕色山羊皮肤组织中的表达量:白色>棕色>黑色;ASIP表达量在白色和黑色山羊皮肤组织之间存在显著差异(P<0.05),但在白色和棕色、棕色和黑色山羊皮肤组织之间差异均不显著(P>0.05)。研究结果提示,Agouti基因在不同毛色山羊皮肤组织中可能存在不同调控机制。 The differential expression of Agouti gene mRNA and its encoding ASIP in goat skin with white,black and brown was detected by SYBR Green real-time PCR and Western blotting respectively in order to investigate the regulation mechanism of Agouti gene on coat color.The results indicated that the expression of mRNA was white>black>brown.There was significant difference of mRNA expression between white and brown(P<0.05),and between black and brown(P<0.05).No significant difference of mRNA expression was found between white and black(P>0.05).The ASIP expression was white>brown>black,and the significant difference of ASIP expression was detected between white and black(P<0.05),no significant difference was found between white and brown,and between brown and black.It could be inferred that there might be different regulatory mechanism of Agoutigene in different skin with different coat color.

关 键 词： 山羊 基因 皮肤组织 差异表达

领　　域： [农业科学] [农业科学]