作　　者： ; ; ; ; ; ; (赵姝君）;

机构地区： 河北农业大学生命科学学院

出　　处： 《畜牧兽医学报》 2014年第9期1417-1423,共7页

摘　　要： 为了确定Meg8基因内部CpG岛的甲基化在调控Meg8基因印记中的可能作用,本研究应用亚硫酸盐测序法分析Meg8基因内含子3上的17个CpGs位点等位基因特异的DNA甲基化状态。结果发现,2条亲本链的甲基化程度都比较高(>80%),但T链甲基化水平显著高于C链(P<0.05),并且C链只有1种甲基化模式。分析Meg8基因在出生后48h死亡的体细胞核移植牛肺中的甲基化和印迹状态,发现Meg8基因甲基化水平在核移植牛和自然繁殖牛中都比较高,但核移植牛的甲基化程度显著高于正常繁殖牛(P<0.05),并且只有1种完全甲基化的模式,而Meg8基因在核移植个体中的印记表达没有紊乱,仍表现为单等位基因表达,初步推测Meg8内含子3CpGs岛的甲基化可能没有参与调控Meg8基因的印记表达。 In order to determine the possible roles of Meg8gene-internal CpG island in the regulation of the gene imprinting expression,the allele-specific DNA methylation status of 17 CpGs in intron3 of Meg8in lung tissues was analyzed using bisulfite sequencing.The results demonstrated that the DNA methylation of both the parental strands were high(>80%),but T-strand showed significantly higher DNA methylation than that of C-strand(P<0.05)and the C-strand had only one kind of methylation model.Then the DNA methylation and imprinting status were analyzed in lung in somatic cell nuclear transfer(SCNT)bovine which died within postnatal 48 h.The results showed that the SCNT bovines exhibited significantly higher DNA methylation than that of naturally breed bovines(P<0.05).All of 17 CpGs showed complete methylation pattern in SCNT bovine.Imprinting analysis showed that Meg8 was mono-allelic expression in tissues of SCNT bovines.The DNA methylation of Meg8intron3 CpGs island may not play a role in regulating of Meg8 imprinting expression.

关 键 词： 体细胞核移植牛 基因 甲基化 印迹

领　　域： [农业科学] [农业科学]