机构地区: 中国科学院上海光学精密机械研究所
出 处: 《物理学报》 2003年第12期3014-3019,共6页
摘 要: 基于麦克斯韦的电磁场理论 ,研究了在全内反射荧光显微术中不同偏振态的入射光所产生的不同偏振的隐失场以及对不同取向的荧光分子荧光发射的影响 .理论分析结果表明 ,p偏振的入射场将产生椭圆偏振的隐失场 ,s偏振的入射场将产生s偏振的隐失场 .随着荧光分子三维取向的不同 ,这两种隐失场的激发荧光效率也将不同 ,由此引起荧光发射强度的各向异性分布 .据此特性 ,可以实现对膜表面分子三维取向的成像 . Based on Maxwell equations, we have discussed the different evanescent fields m ade by excitation with different polarized incident fields, and their influence on fluorescence emissio n. Our analysis showed that the incident light of p-polarization produces a n ellipitically polarized evanescent field; at the same time, the incident light of s-polarization produces a pure s-polarization evanescent field. The probability of the excitation of fluorescent molecules by an evanescent wave var ies with the fluorescent molecules orientation, which will in turn result in th e anisotropic fluorescence emission.