作　　者： ; ; ; ; ;

机构地区： 汕头大学理学院生物系

出　　处： 《花生学报》 2003年第B11期306-310,共5页

摘　　要： 实验比较了11个花生品种在MS和1／2MS培养基中萌发情况，及其在4种芽诱导培养基中的叶段芽诱导率；并观察了70％酒精和0．1％升汞不同处理时间的灭菌效果及其对种子萌发的影响。结果表明：花生种子用酒精处理20-30s和升汞处理10～12min灭菌效果理想，萌发率高；种子萌发率在1／2MS培养基中要比MS培养基高；芽诱导率以汕油523最高，在芽诱导培养基1中为93．1％，汕油31最差，在芽诱导培养基2中仅为9．5％。 We compared germinate status of 11 peanut species on MS medium and 1/2 MS medium, calculated shoot induce rate of different genotype peanut on four mediums, observed sterilizing effect and effect on seed germinate of different disposal time of 75% Alcohol and 0.1% HgCL2.The result indicate: after seeds were deal with Alcohol 20~30s and HgCL2 10~12min, we gained high germinate rate and good sterilize efficiency: all peanut species' germinate rate on 1/2 MS medium higher on MS medium; shoot induce rate of shanyou 523 was 93.1%, the highest on shoot induce medium 1; the worst shoot induce status was shanyou 31, this specie's shoot induce rate only was 9.5% on shoot induce medium 2.

关 键 词： 花生 品种 外植体 芽诱导 培养基 种子萌发 组织培养

领　　域： [农业科学]